SDS-PAGE
CD55 / DAF recombinant protein
Recombinant Rat CD55 / DAF Protein (Fc tag)
Gene Names
Cd55; Daf; Daf1
Purity
> 82 % as determined by SDS-PAGE
Synonyms
CD55 / DAF; Recombinant Rat CD55 / DAF Protein (Fc tag); CD55; DAF; Daf1; CD55 / DAF recombinant protein
Host
Human Cells
Purity/Purification
> 82 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
399
Application Notes
The recombinant rat CD55/Fc is a disulfide-linked homodimer. The reduced monomer comprises 578 amino acids and has a predicted molecular mass of 64.5 kDa. The apparent molecular mass of the protein is approximately 91 and 54 kDa in SDS-PAGE under reducing conditions.
Predicted N Terminal
Asp 35
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for CD55 / DAF recombinant protein
Background: CD55, also well known as decay-accelerating factor (DAF), is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. It is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. CD55 is physiologically acting as an inhibitor of the complement system, but is also broadly expressed in malignant tumours. DAF seems to exert different functions beyond its immunological role such as promotion of tumorigenesis, decrease of complement mediated tumor cell lysis, autocrine loops for cell rescue and evasion of apoptosis, neoangiogenesis, invasiveness, cell motility. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection. This 70-75 kDa glycoprotein CD55 containing four SCR modules is involved in the regulation of the complement cascade. It inhibits complement activation by suppressing the function of C3/C5 convertases, thereby limiting local generation or deposition of C3a/C5a and membrane attack complex (MAC or C5b-9) production. DAF has been identified as a ligand for an activation-associated, seven-transmembrane lymphocyte receptor, CD97, which is a receptor mediating attachment and infection of several viruses and bacteria. In addition, it has been shown that DAF regulates the interplay between complement and T cell immunity in vivo, and thus may be implicated in immune and tumor biology.
Description: A DNA sequence encoding the rat CD55 (Q9Z0M0) (Met1-Ser371) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Description: A DNA sequence encoding the rat CD55 (Q9Z0M0) (Met1-Ser371) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,025 Da
NCBI Official Full Name
complement decay-accelerating factor
NCBI Official Synonym Full Names
CD55 molecule, decay accelerating factor for complement
NCBI Official Symbol
Cd55
NCBI Official Synonym Symbols
Daf; Daf1
NCBI Protein Information
complement decay-accelerating factor
UniProt Protein Name
Cd55 molecule
UniProt Gene Name
Cd55
NCBI Description
accelerates the decay of the classical and alternative pathway C3 convertases, C4b2a and C3bBb [RGD, Feb 2006]
Research Articles on CD55 / DAF
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Product Notes
The CD55 / DAF cd55 (Catalog #AAA2547352) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant rat CD55/Fc is a disulfide-linked homodimer. The reduced monomer comprises 578 amino acids and has a predicted molecular mass of 64.5 kDa. The apparent molecular mass of the protein is approximately 91 and 54 kDa in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the CD55 / DAF cd55 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD55 / DAF, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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