Soluble CD4 Recombinant Protein | CD4 recombinant protein

Mouse Soluble CD4 Recombinant

Cat. Num. AAA553297

• Purity: >90%, as determined by SDS-PAGE and HPLC
• Synonyms: Soluble CD4; Mouse Soluble CD4 Recombinant; Mouse CD4 recombinant; cd4; cd-4; CD4 recombinant protein

• Host: HEk293 Cells
• Purity/Purification: >90%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant CD4 was lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
• Sequence: MNRGVPFRHL LLVLQLALLP AATQGKKVVL GKKGDTVELT CTASQKKSIQ FHWKNSNQIK ILGNQGSFLT KGPSKLNDRA DSRRSLWDQG NFPLIIKNLK IEDSDTYICE VEDQKEEVQL LVFGLTANSD THLLQGQSLT LTLESPPGSS PSVQCRSPRG KNIQGGKTLS VSQLELQDSG TWTCTVLQNQ KKVEFKIDIV VLAFQKASSI VYKKEGEQVE FSFPLAFTVE KLTGSGELWW QAERASSSKS WITFDLKNKE VSVKRVTQDP KLQMGKKLPL HLTLPQALPQ YAGSGNLTLA LEAKTGKLHQ EVNLVVMRAT QLQKNLTCEV WGPTSPKLML SLKLENKEAK VSKREKAVWV LNPEAGMWQC LLSDSGQVLL ESNIKVLPTW STPVQPMALI VLGGVAGLLL FIGLGIFFCV RCRHRRRQAE RMSQIKRLLS EKKTCQCPHR FQKTCSPI
• Sequence Length: 457

• Domain: IG
• Host Note: Optimized DNA sequence encoding Mouse CD4 extracellular domain was expressed in HEK293 cells.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
• Molecular Weight Note: Mouse soluble CD4 is generated by the proteolytic removal of the extracellular domain; and propeptide, the molecule has a calculated molecular mass of approximately 40 kDa. Recombinant mouse CD4 is a disulfide-linked monomer protein consisting of 380 amino acid residue subunits, and migrates due to glycosylation as an approximately 46 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for CD4 recombinant protein

CD4 is a 55 kDa transmembrane glycoprotein belonging to the immunoglobulin superfamily. In humans CD4 is expressed of peripheral T-cells, thymocytes, on macrophages and granulocytes. CD4 acts as a cellular adhesion molecule binding MHC class 2 molecules. It stabilizes the interaction of MHC class 2 restricted T- cells and antigen-presenting cells expressing an antigen in combination with a MHC class 2 molecule. Interactions of CD4 with MHC class 2 molecules are crucial during thymic development and subsequently for the function of single- positive CD4 (+), CD8 (-) T- lymphocytes. CD4 functions as an effector molecule for intracellular signal transduction. In T- lymphocytes CD4 has been found to be associated with tyrosine kinase lck which phosphorylates a number of other cell surface proteins and is involved probably in transducing the intracellular signal when these proteins are bound to their receptors.

Product Categories/Family for CD4 recombinant protein

Receptors CD

References

• influence of novel CD4 binding-defective hiv-1 envelope glycoprotein immunogens on neutralizing antibody and t-cell responses in nonhuman primates .J. Virol., Feb 2010; 84: 1683 - 1695.
• vaccination with an adenoviral vector that encodes and displays a retroviral antigen induces improved neutralizing antibody and CD4+ t-cell responses and confers enhanced protection .J. Virol., Feb 2010; 84: 1967 - 1976.
• defects in il-2r signaling contribute to diminished maintenance of foxp3 expression in CD4+cd25+ regulatory t-cells of type 1 diabetic subjects .Diabetes, Feb 2010; 59: 407 - 415.
• cellular immune responses to severe acute respiratory syndrome coronavirus (sars-cov) infection in senescent balb/c mice: CD4+ t cells are important in control of sars-cov infection .J. Virol., Feb 2010; 84: 1289 - 1301.
• decrease of initial CD4+ t-cell counts at the time of diagnosis of hiv infection in korea; 1988–2006 .Int J STD AIDS, Feb 2010; 21: 120 - 125.