Rabbit anti-Human, Mouse NFKBIA Polyclonal Antibody | anti-NFKBIA antibody

NFKBIA Polyclonal Antibody

Cat. Num. AAA9131480

• Gene Names: NFKBIA; IKBA; MAD-3; NFKBI
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence, Immunoprecipitation
• Purity: Affinity Purification
• Synonyms: NFKBIA; Polyclonal Antibody; NFKBIA Polyclonal Antibody; IKBA; MAD-3; NFKBI; anti-NFKBIA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 317

• Applicable Applications for anti-NFKBIA antibody: Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500 - 1:2000; IF: 1:20 - 1:50; IP: 1:20 - 1:50
• Immunogen: Recombinant protein of human NFKBIA
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using NFKBIA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells using NFKBIA antibody. Blue: DAPI for nuclear staining.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug NFKBIA antibody. Western blot was performed from the immunoprecipitate using NFKBIA antibody at a dilition of 1:500.)

Related Product Information for anti-NFKBIA antibody

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.

Product Categories/Family for anti-NFKBIA antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 126682
• NCBI GeneID: 4792
• UniProt Accession #: P25963; B2R8L6
• Molecular Weight: 36kDa
• NCBI Official Full Name: NF-kappa-B inhibitor alpha
• NCBI Official Synonym Full Names: NFKB inhibitor alpha
• NCBI Official Symbol: NFKBIA
• NCBI Official Synonym Symbols: IKBA; MAD-3; NFKBI
• NCBI Protein Information: NF-kappa-B inhibitor alpha
• UniProt Protein Name: NF-kappa-B inhibitor alpha
• Protein Family: NF-kappa-B inhibitor
• UniProt Gene Name: NFKBIA
• UniProt Synonym Gene Names: IKBA; MAD3; NFKBI; IkB-alpha; IkappaBalpha

NCBI Description

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Uniprot Description

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.