Rabbit CALCRL / CGRP Receptor Polyclonal Antibody | anti-CALCRL antibody

Rabbit Polyclonal to Human CALCRL / CGRP Receptor

Cat. Num. AAA244993

• Gene Names: CALCRL; CRLR; CGRPR
• Reactivity: Human (Predicted Reactivity: Monkey, Mouse, Rabbit)
• Applications: Immunohistochemistry, Immunohistochemistry
• Purity: Immunoaffinity purified
• Synonyms: CALCRL / CGRP Receptor; Polyclonal Antibody; Rabbit Polyclonal to Human CALCRL / CGRP Receptor; Anti-CALCRL / CGRP Receptor Antibody (N-Terminus) IHC-plus; CALCRL; CGRP type 1 receptor; Cgrp1 receptor; CRLR; Calcitonin receptor-like; CGRP receptor; CGRPR; Human CALCRL; CGRP Receptor; anti-CALCRL antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Monkey, Mouse, Rabbit)
• Clonality: Polyclonal
• Specificity: Human CALCRL. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS, 0.1% Sodium Azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CALCRL antibody: Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-Paraffin)
• Application Notes: IHC-Paraffin: 16ug/ml; ; Optimal dilution to be determined by the researcher.
• Target: CALCRL/CGRP Receptor
• Family: GPCR/ Adrenomedullin
• Immunogen Species: CALCRL/CGRP receptor was raised against human.
• Antigen Type: Synthetic peptide
• Immunogen: CALCRL/CGRP Receptor antibody was raised against synthetic 15 amino acid peptide from N-Terminus extracellular domain of human CALCRL. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%); Marmoset, Mouse, Rabbit (93%); Rat, Panda, Dog, Horse, Pig (87%); Goat, Bovine (80%).
• Epitope: N-Terminus
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store undiluted at -20°C or below for up to one year. Can be stored undiluted at 4°C for up to one month. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-CALCRL / CGRP Receptor antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-CALCRL / CGRP Receptor antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-CALCRL / CGRP Receptor antibody IHC of human Bone, Osteosarcoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-CALCRL antibody IHC of human colon, submucosal vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-CALCRL antibody

Calcitonin receptor-like receptor (CRLR) is an Adrenomedullin Receptor that requires two additional proteins to function: a chaperone protein RAMP (receptor activity modifying protein) and the component protein RCP. The function of CRLR depends on its interaction with RAMP. When coexpressed with RAMP1, CRLR acts as a receptor for calcitonin gene-related peptide (CGRP). In contrast, when CRLR is coexpressed with either RAMP2 or RAMP3, it acts as a receptor for adrenomedullin.

Product Categories/Family for anti-CALCRL antibody

Family: GPCR; Subfamily: Adrenomedullin

NCBI and Uniprot Product Information

• NCBI GI #: 5031621
• NCBI GeneID: 10203
• NCBI Accession #: NP_005786.1
• NCBI GenBank Nucleotide #: NM_005795.5
• UniProt Accession #: Q16602; Q53S02; Q53TS5; A8K6G5; A8KAD3
• NCBI Official Full Name: calcitonin gene-related peptide type 1 receptor
• NCBI Official Synonym Full Names: calcitonin receptor-like
• NCBI Official Symbol: CALCRL
• NCBI Official Synonym Symbols: CRLR; CGRPR
• NCBI Protein Information: calcitonin gene-related peptide type 1 receptor; CGRP type 1 receptor; calcitonin receptor-like receptor
• UniProt Protein Name: Calcitonin gene-related peptide type 1 receptor
• Protein Family: Calcitonin gene-related peptide type 1 receptor
• UniProt Gene Name: CALCRL
• UniProt Synonym Gene Names: CGRPR; CGRP type 1 receptor
• UniProt Entry Name: CALRL_HUMAN

Uniprot Description

CALCRL: Receptor for calcitonin-gene-related peptide (CGRP) together with RAMP1 and receptor for adrenomedullin together with RAMP3. Receptor for adrenomedullin together with RAMP2. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 2 family.

Protein type: Receptor, GPCR; Membrane protein, integral; Cell cycle regulation; Membrane protein, multi-pass; GPCR, family 2

Chromosomal Location of Human Ortholog: 2q32.1

Cellular Component: integral to plasma membrane; lysosome; endoplasmic reticulum; plasma membrane; endosome

Molecular Function: G-protein coupled receptor activity; protein binding; calcitonin gene-related polypeptide receptor activity; adrenomedullin receptor activity; protein transporter activity; calcitonin receptor activity

Biological Process: G-protein signaling, coupled to cyclic nucleotide second messenger; protein transport; regulation of muscle contraction; negative regulation of inflammatory response; heart development; positive regulation of smooth muscle cell proliferation; receptor internalization; calcium ion transport; positive regulation of cAMP biosynthetic process; cAMP biosynthetic process; angiogenesis; G-protein signaling, adenylate cyclase activating pathway