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Western Blot (WB)
(Mouse Map2k1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the Map2k1 antibody detected the Map2k1 protein (arrow).)
Rabbit anti-Human, Mouse Map2k1 Polyclonal Antibody | anti-Map2k1 antibody
Map2k1, CT (Map2k1, Mek1, Prkmk1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAPK/ERK kinase 1) (AP)
Gene Names
Map2k1; Mek1; MEKK1; MAPKK1; Prkmk1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Map2k1; Polyclonal Antibody; CT (Map2k1; Mek1; Prkmk1; Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1) (AP); MAPK/ERK kinase 1; anti-Map2k1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-Map2k1 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
Mouse Map2k1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 268-296 amino acids from the C-terminal region of Mouse Map2k1.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Mouse Map2k1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the Map2k1 antibody detected the Map2k1 protein (arrow).)
Western Blot (WB)
(Mouse Map2k1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the Map2k1 antibody detected the Map2k1 protein (arrow).)
Western Blot (WB)
(Western blot analysis of Map2k1 (arrow) using rabbit polyclonal Mouse Map2k1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map2k1 gene.)
Western Blot (WB)
(Western blot analysis of Map2k1 (arrow) using rabbit polyclonal Mouse Map2k1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map2k1 gene.)
Western Blot (WB)
(Mouse Map2k1 Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the Map2k1 antibody detected the Map2k1 protein (arrow).)
Western Blot (WB)
(Mouse Map2k1 Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the Map2k1 antibody detected the Map2k1 protein (arrow).)
Immunohistochemistry (IHC)
(Mouse Map2k1 Antibody (C-term) (AP14153b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map2k1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Mouse Map2k1 Antibody (C-term) (AP14153b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map2k1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-Map2k1 antibody
Map2k1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Product Categories/Family for anti-Map2k1 antibody
References
Chandrakesan, P., et al. J. Biol. Chem. 285(43):33485-33498(2010). Yang, T., et al. J. Clin. Invest. 120(7):2474-2485(2010). Cho, K.W., et al. J. Exp. Zool. B Mol. Dev. Evol. 312(8):901-911(2009). Jo, C., et al. Cell. Signal. 21(12):1910-1917(2009). Mandal, M., et al. Nat. Immunol. 10(10):1110-1117(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,474 Da
NCBI Official Full Name
dual specificity mitogen-activated protein kinase kinase 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase 1
NCBI Official Symbol
Map2k1
NCBI Official Synonym Symbols
Mek1; MEKK1; MAPKK1; Prkmk1
NCBI Protein Information
dual specificity mitogen-activated protein kinase kinase 1; MEK 1; MAPKK 1; MAPK/ERK kinase 1; MAP kinase kinase 1; ERK activator kinase 1; mitogen activated protein kinase kinase 1; protein kinase, mitogen activated, kinase 1, p45
UniProt Protein Name
Dual specificity mitogen-activated protein kinase kinase 1
UniProt Gene Name
Map2k1
UniProt Synonym Gene Names
Mek1; Prkmk1; MAP kinase kinase 1; MAPKK 1; MEK 1
UniProt Entry Name
MP2K1_MOUSE
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Product Notes
The Map2k1 map2k1 (Catalog #AAA6318440) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Map2k1, CT (Map2k1, Mek1, Prkmk1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAPK/ERK kinase 1) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Map2k1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Map2k1 map2k1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Map2k1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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