SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
B7-H3 recombinant protein
Recombinant B7-H3
Gene Names
Cd276; B7h3; B7RP-2; AU016588; 6030411F23Rik
Applications
SDS-Page
Purity
>90% by SDS-PAGE
Synonyms
B7-H3; Recombinant B7-H3; CD276 antigen; Cd276; 6030411F23Rik; AU016588; B7h3; B7RP-2; B7 homolog 3; B7-H3 recombinant protein
Host
Baculovirus
Purity/Purification
>90% by SDS-PAGE
Form/Format
Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 10% Glycerol.
Concentration
0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
29-248aa
Sequence
VEVQVSEDPV VALVDTDATL RCSFSPEPGF SLAQLNLIWQ LTDTKQLVHS FTEGRDQGSA YSNRTALFPD LLVQGNASLR LQRVRVTDEG SYTCFVSIQD FDSAAVSLQV AAPYSKPSMT LEPNKDLRPG NMVTITCSSY QGYPEAEVFW KDGQGVPLTG NVTTSQMANE RGLFDVHSVL RVVLGANGTY SCLVRNPVLQ QDAHGSVTIT GQPLTFPPEA HHHHHH
Sequence Length
316
Applicable Applications for B7-H3 recombinant protein
SDS-PAGE
Species
Mouse
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Predicted N-Terminal
Val 29
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for B7-H3 recombinant protein
B7H3, also known as CD276 antigen, is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It modulates T-cell-mediated immune responses and the development of acute and chronic transplant rejection. This protein plays a positive regulatory role in bone formation and has a dual role in the bone-immune interface. It induces antitumor immunity as it activates both acquired and innate immunity leading to natural killer cell and CD8 T-cell dependent killing of tumor cells. Recombinant mouse B7H3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for B7-H3 recombinant protein
References
Wang Z., et al. (2018) Cancer Sci. 109:2697-2705. Son Y., et al. (2019) Stem Cells. 37:382-394.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24.7kDa (226aa)
NCBI Official Full Name
CD276 antigen
NCBI Official Synonym Full Names
CD276 antigen
NCBI Official Symbol
Cd276
NCBI Official Synonym Symbols
B7h3; B7RP-2; AU016588; 6030411F23Rik
NCBI Protein Information
CD276 antigen
UniProt Protein Name
CD276 antigen
UniProt Gene Name
Cd276
UniProt Synonym Gene Names
B7h3
UniProt Entry Name
CD276_MOUSE
Research Articles on B7-H3
Similar Products
Product Notes
The B7-H3 cd276 (Catalog #AAA201155) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 29-248aa. AAA Biotech's B7-H3 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the B7-H3 cd276 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: VEVQVSEDPV VALVDTDATL RCSFSPEPGF SLAQLNLIWQ LTDTKQLVHS FTEGRDQGSA YSNRTALFPD LLVQGNASLR LQRVRVTDEG SYTCFVSIQD FDSAAVSLQV AAPYSKPSMT LEPNKDLRPG NMVTITCSSY QGYPEAEVFW KDGQGVPLTG NVTTSQMANE RGLFDVHSVL RVVLGANGTY SCLVRNPVLQ QDAHGSVTIT GQPLTFPPEA HHHHHH. It is sometimes possible for the material contained within the vial of "B7-H3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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