Western Blot (WB)
Rabbit SGLT1 Polyclonal Antibody | anti-SGLT1 antibody
SGLT1 Antibody
ELISA: 1:4,000
Immunocytochemistry: 1:250
Immunofluorescence: 1:250
Immunohistochemistry: 1:250
Immunoprecipitation: 1:200
Western Blot: 1:500-1:1,000
Western Blot (WB)
Western Blot (WB)
Overview:
Sodium-glucose linked transporters (SLGT) are a family of glucose transporters that contribute to renal glucose reabsorption. The two most common members of SGLT family are members of the SLC5A gene family : SGLT1 and SGLT2. There are five other members in the human protein family SLC5Aa, which may also be sodium-glucose transporters (1). SGLTs utilize secondary active transport to transport glucose along kidney proximal tubules by sodium ion cotransport with a sodium ion to glucose coupling ration of 2:1. A. Na+/K+ ATPase pump on the basolateral membrane of the proximal tubules cell utilizes ATP to move 3 Sodium ions toward into the blood, and 2 potassium ions into the cell. The energy from the resulting downhill sodium ion gradient is utilized by SGLTs to transport glucose across the apical membrane. Since sodium and glucose are transported in the same direction SGLTs are classified as symporters. SGLTs are mainly expressed in intestine and kidney.
SGLT and GLUT antibodies have been fully characterized for cross reactivity within the SGLT and GLUT families and also with other cellular proteins. Antibodies against multiple epitopes on the same protein to facilitate studies using interspecies cross reactivity. SGLT western blot positive control( PC-SGLT) is available in ready-to use SDS-PAGE sample buffer. The SGLT positive control appears as a diffuse band of 69-72 and co-migrates with rat jejunal sodium-dependent glucose transporter protein on a 10% SDS-PAGE.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Uniprot Description
SLC5A1: Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. Expressed mainly in intestine and kidney. Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
Protein type: Transporter; Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral
Chromosomal Location of Human Ortholog: 22q12.3
Cellular Component: apical plasma membrane; brush border membrane; integral to plasma membrane; intercellular junction; plasma membrane
Molecular Function: glucose:sodium symporter activity; protein binding
Biological Process: D-glucose absorption; glucose transport; hexose transport; sodium ion transport
Disease: Glucose/galactose Malabsorption
Research Articles on SGLT1
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Product Notes
The SGLT1 slc5a1 (Catalog #AAA540266) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SGLT1 Antibody reacts with Human, Mouse, Rat. and may cross-react with other species as described in the data sheet. AAA Biotech's SGLT1 can be used in a range of immunoassay formats including, but not limited to, Confocal Microscopy (CM), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). Dot Blot: 1:4,000 ELISA: 1:4,000 Immunocytochemistry: 1:250 Immunofluorescence: 1:250 Immunohistochemistry: 1:250 Immunoprecipitation: 1:200 Western Blot: 1:500-1:1,000. Researchers should empirically determine the suitability of the SGLT1 slc5a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SGLT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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