CD24 recombinant protein

CD24 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120639

• Gene Names: CD24; CD24A; FLJ22950; FLJ43543; MGC75043
• Purity: >92% as determined by SDS-PAGE
• Synonyms: CD24; CD24 Protein; Human; Recombinant (hFc Tag); Human CD24 Protein (Fc Tag); CD24A Protein; FLJ22950 Protein; FLJ43543 Protein; MGC75043 Protein; CD24 molecule; CD24 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly59

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 27
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human CD24 (NP_037362.1)(Met 1-Gly 59) was fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD24 recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 24, also known as signal transducer CD24 or heat stable antigen CD24 (HSA), is a mucin-type glycosylphosphatidylinositol-linked glycoprotein expressed on the surface of B-cells, differentiating neuroblasts and many tumors. It is involved in molecular adhesion and metastatic tumor spread and serve as a normal receptor for P-selectin. The CD24/P-selectin pathway could be important in disseminating tumor cells by facilitating the interaction with platelet and endothelial cells. It has also been considered as a tumor marker. High rate of CD24 expressions have been found in epithelial ovarian cancer, breast cancer, non-small cell lung cancer, prostate cancer and pancreatic cancer.

Product Categories/Family for CD24 recombinant protein

Cancer Drug Targets

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.Kristiansen G, et al. (2003) Tumour biological aspects of CD24, a mucin-like adhesion molecule. Journal of molecular histology. 35 (3): 255-62.

NCBI and Uniprot Product Information

• NCBI GI #: 7019343
• NCBI GeneID: 100133941
• NCBI Accession #: NP_037362.1
• NCBI GenBank Nucleotide #: NM_013230.2
• UniProt Accession #: P25063; Q16257; Q53XS0
• Molecular Weight: 8,097 Da
• NCBI Official Full Name: signal transducer CD24
• NCBI Official Synonym Full Names: CD24 molecule
• NCBI Official Symbol: CD24
• NCBI Official Synonym Symbols: CD24A; FLJ22950; FLJ43543; MGC75043
• NCBI Protein Information: signal transducer CD24; CD24 antigen (small cell lung carcinoma cluster 4 antigen)
• UniProt Protein Name: Signal transducer CD24
• Protein Family: Signal transducer
• UniProt Gene Name: CD24
• UniProt Synonym Gene Names: CD24A
• UniProt Entry Name: CD24_HUMAN

Uniprot Description

CD24: Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS). A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface. Belongs to the CD24 family.

Protein type: Membrane protein, GPI anchor; Cell surface; Cell development/differentiation; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cell surface; membrane; plasma membrane; lipid raft

Molecular Function: protein binding; signal transducer activity; protein tyrosine kinase activator activity; protein kinase binding

Biological Process: respiratory burst; axon guidance; cell migration; Wnt receptor signaling pathway; regulation of phosphorylation; B cell receptor transport into lipid raft; immune response-regulating cell surface receptor signaling pathway; chemokine receptor transport out of lipid raft; positive regulation of activated T cell proliferation; positive regulation of MAP kinase activity; cell activation; cholesterol homeostasis; cell-cell adhesion; elevation of cytosolic calcium ion concentration; response to estrogen stimulus; response to molecule of bacterial origin; regulation of MAPKKK cascade; negative regulation of transforming growth factor-beta3 production; T cell costimulation; regulation of epithelial cell differentiation; response to hypoxia; regulation of cytokine and chemokine mediated signaling pathway

Disease: Multiple Sclerosis, Susceptibility To