Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse CD209b expression on mouse splenocytes. BABL/c splenocytes were stained with PE-conjugated anti-Mouse CD209b and PerCP-Cy'5.5 conjugated anti-Mouse CD19 (BD Pharmingen'). The dot plots were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Rabbit anti-Mouse SIGNR1 Monoclonal Antibody | anti-CD209B antibody
Recombinant Anti-SIGNR1 Antibody (PE), Rabbit Monoclonal
Gene Names
Cd209b; OtB7; SIGNR1; mSIGNR1; DC-SIGNR1; 1810030I22Rik
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
SIGNR1; Monoclonal Antibody; Recombinant Anti-SIGNR1 Antibody (PE); Rabbit Monoclonal; SIGNR1/CD209b Antibody (PE); Rabbit MAb; CD209b antigen; Anti-1810030I22Rik Antibody; Anti-DC-SIGNR1 Antibody; Anti-mSIGNR1 Antibody; Anti-OtB7 Antibody; Anti-SIGNR1 Antibody; anti-CD209B antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
143
Specificity
Mouse SIGNR1/CD209b
Purity/Purification
Protein A
Form/Format
Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
10ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-CD209B antibody
Flow Cytometry (FC/FACS/FCM)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse SIGNR1/CD209b protein
Conjugation
PE
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse SIGNR1/CD209b (rM SIGNR1/CD209b) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse CD209b expression on mouse splenocytes. BABL/c splenocytes were stained with PE-conjugated anti-Mouse CD209b and PerCP-Cy'5.5 conjugated anti-Mouse CD19 (BD Pharmingen'). The dot plots were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse CD209b expression on mouse splenocytes. BABL/c splenocytes were stained with PE-conjugated anti-Mouse CD209b and PerCP-Cy'5.5 conjugated anti-Mouse CD19 (BD Pharmingen'). The dot plots were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Related Product Information for anti-CD209B antibody
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD209b, also known as SIGNR1, is a C-type lectin receptor. CD209b is present on most regions of mouse brain and found on microglia and dendritic cells but not on neurons or astrocytes. CD209b is implicated in the recently described SIGNR1 complement activation pathway, which operates against capsular polysaccharides in splenic marginal macrophages. CD209b in rat is homologue to SIGNR1 in mouse, both of which are found to mediate the uptake of dextran or CPS14 within the splenic marginal zone.
References
Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.Park JY, et al. (2009) The C-type lectin CD209b is expressed on microglia and it mediates the uptake of capsular polysaccharides of Streptococcus pneumonia. Neuroscience Letters. 450 (3): 246-51.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,471 Da
NCBI Official Full Name
CD209 antigen-like protein B isoform a
NCBI Official Synonym Full Names
CD209b antigen
NCBI Official Symbol
Cd209b
NCBI Official Synonym Symbols
OtB7; SIGNR1; mSIGNR1; DC-SIGNR1; 1810030I22Rik
NCBI Protein Information
CD209 antigen-like protein B
UniProt Protein Name
CD209 antigen-like protein B
Protein Family
UniProt Gene Name
Cd209b
UniProt Synonym Gene Names
DC-SIGNR1
UniProt Entry Name
C209B_MOUSE
Uniprot Description
CD209B: Probable pathogen-recognition receptor. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. May recognize in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: membrane; integral to membrane; external side of plasma membrane
Molecular Function: mannose binding; metal ion binding; zymosan binding; carbohydrate binding; polysaccharide binding
Biological Process: positive regulation of tumor necrosis factor biosynthetic process; detection of bacterium; positive regulation of phagocytosis; endocytosis; phagocytosis, recognition; detection of yeast
Research Articles on CD209B
Similar Products
Product Notes
The CD209B cd209b (Catalog #AAA8111647) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-SIGNR1 Antibody (PE), Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SIGNR1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). Researchers should empirically determine the suitability of the CD209B cd209b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIGNR1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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