IL-32 ELISA Pair Set | IL32 elisa pair set

IL-32 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118344

• Synonyms: IL-32; IL-32 Matched ELISA Antibody Pair Set; Human; Human Interleukin-32/IL-32 ELISA Pair Set; interleukin 32; IL-32alpha Matched ELISA Antibody Pair Set; IL-32beta Matched ELISA Antibody Pair Set; IL-32delta Matched ELISA Antibody Pair Set; IL-32gamma Matched ELISA Antibody Pair Set; IL32 Matched ELISA Antibody Pair Set; NK4 Matched ELISA Antibody Pair Set; TAIF Matched ELISA Antibody Pair Set; TAIFa Matched ELISA Antibody Pair Set; TAIFb Matched ELISA Antibody Pair Set; TAIFc Matched ELISA Antibody Pair Set; TAIFd Matched ELISA Antibody Pair Set; IL32 elisa pair set

• Specificity: Quantitative determination of Human IL-32

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 23.44-1500pg/mL
• Sensitivity: 23.4375pg/ml
• Materials Provided: 1. Capture Antibody 1.0 mg/mL of rabbit anti-IL32A monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL rabbit anti-IL32A monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 33 ng of recombinant IL32A. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1.5ng/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for IL32 elisa pair set

IL-32 is a recently discovered cytokine that induces various proinflammatory cytokines (TNF-alpha, IL-1beta, IL-6) and chemokines in both human and mouse cells through the NF-kappaB and p38 MAPK inflammatory signal pathways. It is regulated robustly by other major proinflammatory cytokines and is crucial to inflammation and immune responses. Four of the IL-32 isoforms (alpha, beta, gamma, and delta) are the most representative IL-32 transcripts, and the gamma isoform of IL-32 is the most active, although all isoforms are biologically active. IL-32, a cytokine produced mainly by T, natural killer, and epithelial cells induces significant amounts of TNFalpha and MIP-2 and increases the production of both cytokines in a dose-dependent manner. IL-32 has been implicated in inflammatory disorders, Mycobacterium tuberculosis infections, inflammatory bowel disease, and influenza A virus infection, as well as in some autoimmune diseases, such as rheumatoid arthritis, ulcerative colitis, and in the human stomach cancer, human lung cancer, and breast cancer tissues. Thus, IL-32 expression might be valuable as a biomarker for cancer.

This IL-32 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human IL-32. It contains Human IL-32 capture antibody, Human IL-32 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human IL-32 protein.

References

Felaco P, et al. (2009) IL-32: a newly-discovered proinflammatory cytokine. J Biol Regul Homeost Agents. 23(3): 141-7.Kobayashi H, et al. (2009) Molecular characterization of IL-32 in human endothelial cells. Cytokine. 46(3): 351-8.Meyer N, et al. (2010) IL-32 is expressed by human primary keratinocytes and modulates keratinocyte apoptosis in atopic dermatitis. J Allergy Clin Immunol. 125(4): 858-865.