c-Kit ELISA Pair Set | KIT elisa pair set

c-Kit Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118417

• Synonyms: c-Kit; c-Kit Matched ELISA Antibody Pair Set; Human; Human KIT/c-KIT/CD117 ELISA Pair Set; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; C-Kit Matched ELISA Antibody Pair Set; CD117 Matched ELISA Antibody Pair Set; PBT Matched ELISA Antibody Pair Set; SCFR Matched ELISA Antibody Pair Set; KIT elisa pair set

• Specificity: Quantitative determination of Human c-Kit

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 23.44-1500pg/mL
• Sensitivity: 23.4375pg/ml
• Materials Provided: 1. Capture Antibody 0.2 mg/mL of rabbit anti-KIT monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL mouse anti-KIT monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 90 ng of recombinant KIT. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1.5ng/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for KIT elisa pair set

C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family, and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumor in histological tissue sections. It is used primarily in the identification of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the identification of mast cell tumors and in distinguishing seminomas from embryonal carcinomas. Mutations in the c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.

This c-Kit Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human c-Kit. It contains Human c-Kit capture antibody, Human c-Kit detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human c-Kit protein.

Product Categories/Family for KIT elisa pair set

Cancer Drug Targets Cardiac Stem Cell Markers Receptor Tyrosine Kinases (RTKs)

References

Andre C, et al. (1997) Sequence analysis of two genomic regions containing the KIT and the FMS receptor tyrosine kinase genes. Genomics. 39(2):216-26.
Yarden Y, et al. (1987) Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J. 6(11):3341-51.
Leong KG, et al. (2008) Generation of a prostate from a single adult stem cell. Nature. 456(7223): 804-8.
Edling CE, et al. (2007) c-Kit--a hematopoietic cell essential receptor tyrosine kinase. Int J Biochem Cell Biol. 39(11):1995-8.
McIntyre A, et al. (2005) Amplification and overexpression of the KIT gene is associated with progression in the seminoma subtype of testicular germ cell tumors of adolescents and adults. Cancer Res. 65(18):8085-9.