General CS (Chondroitin Sulfate) ELISA Kit | CS elisa kit

CS (Chondroitin Sulfate) ELISA Kit

Cat. Num. AAA2540387

• Reactivity: General
• Synonyms: CS (Chondroitin Sulfate); CS (Chondroitin Sulfate) ELISA Kit; CS elisa kit

• Reactivity: General
• Specificity: This kit recognizes Cortisol in samples. No significant cross-reactivity or interference between Cortisol and analogues was observed.

• Samples: Porcine Serum And Plasma
• Assay Type: Quantitative Competitive
• Detection Range: 0.78-50ng/mL
• Sensitivity: 0.48ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 porcine samples with low, mid range and high level Cortisol were tested 20 times on one plate, respectively
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 porcine samples with low, mid range and high level Cortisol were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CS elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Cortisol concentrations in Porcine serum and plasma. Please contact tech-support for other sample type detection.

Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Cortisol. During the reaction, Cortisol in the sample or standard competes with a fixed amount of Cortisol on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve!!Background/Introduction: Cortisol is often called the "stress hormone" because of its connection to the stress response, however, cortisol is much more than just a hormone released during stress. Cortisol is one of the steroid hormones and is made in the adrenal glands. Secretion of the hormone is controlled by the hypothalamus, the pituitary gland, and the adrenal gland, a combination glands often referred to as the HPA axis. Because most bodily cells have cortisol receptors, it affects many different functions in the body. Cortisol can help control blood sugar levels, regulate metabolism, [1] help reduce inflammation, and assist with memory formulation. It has a controlling effect on salt and water balance and helps control blood pressure. In women, cortisol also supports the developing fetus during pregnancy. Low cortisol levels can cause a condition known as primary adrenal insufficiency or Addison disease. While rare, primary adrenal insufficiency is an autoimmune disease that causes damage to the adrenal glands Cortisol reduces bone formation, favoring long-term development of osteoporosis. It transports potassium out of cells in exchange for an equal number of sodiumions. This can trigger the hyperkalemia of metabolic shock from surgery. Cortisol also reduces calcium absorption in the intestine [2].