Western Blot (WB)
(Western Blot: Sample: Human U87MG cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Bovine CCND1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Cattle Cyclin D1 (CCND1) Polyclonal Antibody | anti-CCND1 antibody
Polyclonal Antibody to Cyclin D1 (CCND1)
Reactivity
Cattle
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Cyclin D1 (CCND1); Polyclonal Antibody; Polyclonal Antibody to Cyclin D1 (CCND1); anti-CCND1 antibody
Host
Rabbit
Reactivity
Cattle
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CCND1. It has been selected for its ability to recognize CCND1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-KCEEEVFPL AMNYLDRFLS LEPVKKSRLQ LLGATCMFVA SKMKETIPLT AEKLCIYTDN SIRPDELLHM ELVLVNKLKW NLAAMTPHDF IEHFLSKMPV AEENKQIIRK HAQTFVALCA TDVKFISNPP SMVAAGSVAA AAQGLHLGSA NGFLSYHRLT RFLSKVIRCD PDCLRACQEQ IEALLESSLR QAQQQNLDPK AA
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-KCEEEVFPL AMNYLDRFLS LEPVKKSRLQ LLGATCMFVA SKMKETIPLT AEKLCIYTDN SIRPDELLHM ELVLVNKLKW NLAAMTPHDF IEHFLSKMPV AEENKQIIRK HAQTFVALCA TDVKFISNPP SMVAAGSVAA AAQGLHLGSA NGFLSYHRLT RFLSKVIRCD PDCLRACQEQ IEALLESSLR QAQQQNLDPK AA
Sequence Length
125
Applicable Applications for anti-CCND1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant CCND1 (Lys72~Ala272) expressed in E.coli.
Cross Reactivity
Human, Bovine
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2044571
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Human U87MG cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Bovine CCND1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human U87MG cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Bovine CCND1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,407 Da
NCBI Official Full Name
G1/S-specific cyclin-D1
NCBI Official Synonym Full Names
cyclin D1
NCBI Official Symbol
CCND1
NCBI Protein Information
G1/S-specific cyclin-D1
UniProt Protein Name
G1/S-specific cyclin-D1
Protein Family
UniProt Gene Name
CCND1
Uniprot Description
Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner ().
Research Articles on CCND1
Similar Products
Product Notes
The CCND1 ccnd1 (Catalog #AAA2001751) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cyclin D1 (CCND1) reacts with Cattle and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin D1 (CCND1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CCND1 ccnd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-KCE EEVFPL AMNYLDRFLS LEPVKKSRLQ LLGATCMFVA SKMKETIPLT AEKLCIYTDN SIRPDELLHM ELVLVNKLKW NLAAMTPHDF IEHFLSKMPV AEENKQIIRK HAQTFVALCA TDVKFISNPP SMVAAGSVAA AAQGLHLGSA NGFLSYHRLT RFLSKVIRCD PDCLRACQEQ IEALLESSLR QAQQQNLDPK AA. It is sometimes possible for the material contained within the vial of "Cyclin D1 (CCND1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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