Typical Testing Data/Standard Curve (for reference only)
Human C C motif chemokine 7 (CCL7/MCP3/SCYA6/SCYA7) ELISA Kit | CCL7 elisa kit
Human C C motif chemokine 7 (CCL7/MCP3/SCYA6/SCYA7) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CCL7/MCP3/SCYA6/SCYA7 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CCL7/MCP3/SCYA6/SCYA7 antibody and an CCL7/MCP3/SCYA6/SCYA7-HRP conjugate. The assay sample and buffer are incubated together with CCL7/MCP3/SCYA6/SCYA7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCL7/MCP3/SCYA6/SCYA7 concentration since CCL7/MCP3/SCYA6/SCYA7 from samples and CCL7/MCP3/SCYA6/SCYA7-HRP conjugate compete for the anti-CCL7/MCP3/SCYA6/SCYA7 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCL7/MCP3/SCYA6/SCYA7 from the sample, fewer sites are left to bind CCL7/MCP3/SCYA6/SCYA7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCL7/MCP3/SCYA6/SCYA7 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q. [provided by RefSeq, Jul 2008]
Uniprot Description
CCL7: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 17q11.2-q12
Cellular Component: extracellular space; extracellular region
Molecular Function: heparin binding; CCR1 chemokine receptor binding; chemokine activity; CCR2 chemokine receptor binding
Biological Process: cellular calcium ion homeostasis; monocyte chemotaxis; regulation of cell shape; cell-cell signaling; eosinophil chemotaxis; response to gamma radiation; cytoskeleton organization and biogenesis; immune response; chemotaxis; inflammatory response; signal transduction; positive regulation of cell migration
Research Articles on CCL7
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Product Notes
The Human CCL7 ccl7 (Catalog #AAA7231354) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7231354 ELISA Kit recognizes Human CCL7. It is sometimes possible for the material contained within the vial of "C C motif chemokine 7 (CCL7/MCP3/SCYA6/SCYA7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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