Human chemokine (C-C motif) ligand 28 ELISA Kit | CCL28 elisa kit

Human mucosae associated epithelia chemokine, MEC ELISA Kit

Cat. Num. AAA705112

• Gene Names: CCL28; MEC; CCK1; SCYA28
• Reactivity: Human
• Synonyms: chemokine (C-C motif) ligand 28; Human mucosae associated epithelia chemokine; MEC ELISA Kit; CCK1; MEC; MGC71902; SCYA28; CC chemokine CCL28; mucosae-associated epithelial chemokine; small inducible cytokine A28; small inducible cytokine subfamily A (Cys-Cys); member 28; CCL28 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human MEC. No significant cross-reactivity or interference between human MEC and analogues was observed.
• Sequence Length: 127

• Samples: Serum, plasma, tissue homogenates.
• Assay Type: Sandwich (Quantitative)
• Detection Range: 47 pg/ml -3000 pg/ml.
• Sensitivity: 11.75 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Related Product Information for CCL28 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MEC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MEC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MEC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEC bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 22538811
• NCBI GeneID: 56477
• NCBI Accession #: NP_683513.1
• NCBI GenBank Nucleotide #: NM_148672.2
• UniProt Accession #: Q9NRJ3; D7RIE7
• Molecular Weight: 14,280 Da
• NCBI Official Full Name: C-C motif chemokine 28
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 28
• NCBI Official Symbol: CCL28
• NCBI Official Synonym Symbols: MEC; CCK1; SCYA28
• NCBI Protein Information: C-C motif chemokine 28; CC chemokine CCL28; small inducible cytokine A28; small-inducible cytokine A28; mucosae-associated epithelial chemokine; chemokine (C-C motif) ligand 28 splice variant chi; small inducible cytokine subfamily A (Cys-Cys), member 28
• UniProt Protein Name: C-C motif chemokine 28
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL28
• UniProt Synonym Gene Names: SCYA28; MEC
• UniProt Entry Name: CCL28_HUMAN

NCBI Description

This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for resting CD4 or CD8 T cells and eosinophils. The product of this gene binds to chemokine receptors CCR3 and CCR10. This chemokine may play a role in the physiology of extracutaneous epithelial tissues, including diverse mucosal organs. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner.