CCL23 recombinant protein

Recombinant Human CCL23 Protein

Cat. Num. AAA8248675

• Gene Names: CCL23; CKb8; MIP3; Ckb-8; MIP-3; MPIF-1; SCYA23; Ckb-8-1; hmrp-2a; CK-BETA-8
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 98.0% as determined by RP-HPLC. Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: CCL23; Recombinant Human CCL23 Protein; MIP3; MPIF1; SCYA23; C-C motif chemokine 23; CK-beta-8; CKB-8; Macrophage inflammatory protein 3; MIP-3; Myeloid progenitor inhibitory factor 1; MPIF-1; Small-inducible cytokine A23; CCL23 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 98.0% as determined by RP-HPLC. Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM PB, 250mM NaCl, pH 7.2.
• Sequence: MRVTKDAETE FMMSKLPLEN PVLLDRFHAT SADCCISYTP RSIPCSLLES YFETNSECSK PGVIFLTKKG RRFCANPSDK QVQVCVRMLK LDTRIKTRKN
• Sequence Length: 137

• Applicable Applications for CCL23 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for CCL23 recombinant protein

Recombinant Human CCL23 Protein is produced by E Coli. It is a single non-glycosylated polypeptide chain containing 100 amino acids with an extra N-terminal Met with a molecular mass of 11.5kD.

NCBI and Uniprot Product Information

• NCBI GI #: 632794829
• NCBI GeneID: 6368
• NCBI Accession #: NP_005055.3
• NCBI GenBank Nucleotide #: NM_005064.5
• UniProt Accession #: P55773; O00174; O75950; Q52LD4; B7ZKQ3
• Molecular Weight: 15,290 Da
• NCBI Official Full Name: C-C motif chemokine 23 isoform CKbeta8-1
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 23
• NCBI Official Symbol: CCL23
• NCBI Official Synonym Symbols: CKb8; MIP3; Ckb-8; MIP-3; MPIF-1; SCYA23; Ckb-8-1; hmrp-2a; CK-BETA-8
• NCBI Protein Information: C-C motif chemokine 23
• UniProt Protein Name: C-C motif chemokine 23
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL23
• UniProt Synonym Gene Names: MIP3; MPIF1; SCYA23; CKB-8; MIP-3; MPIF-1
• UniProt Entry Name: CCL23_HUMAN

NCBI Description

This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils and no activity on activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. In addition, the product of this gene is a potent agonist of the chemokine (C-C motif) receptor 1. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

CCL6: Shows chemotactic activity for monocytes, resting T- lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular region; extracellular space

Molecular Function: CCR1 chemokine receptor binding; chemokine activity

Biological Process: cell-cell signaling; cellular calcium ion homeostasis; chemotaxis; G-protein coupled receptor protein signaling pathway; immune response; lymphocyte chemotaxis; monocyte chemotaxis; negative regulation of cell proliferation; neutrophil chemotaxis; positive regulation of GTPase activity; positive regulation of inflammatory response