Monocyte Chemotactic Protein-1 (CCL2) Recombinant Protein | CCL2 recombinant protein

Recombinant Rat Monocyte Chemotactic Protein-1 (CCL2), Sf9

Cat. Num. AAA147321

• Gene Names: Ccl2; MCP-1; Scya2; Sigje
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Monocyte Chemotactic Protein-1 (CCL2); Recombinant Rat Monocyte Chemotactic Protein-1 (CCL2); Sf9; Monocyte Chemotactic Protein-1 Rat Recombinant; Small inducible cytokine A2; CCL2; Monocyte chemotactic protein 1; MCP-1; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; MCAF; Monocyte secretory protein JE; HC11; chemokine (C-C motif) ligand 2; MCP1; SCYA2; GDCF-2; SMC-CF; HSMCR30; MGC9434; GDCF-2 HC11; MCP1 Rat; rMCP1; CCL2 recombinant protein

• Host: Sf9, Baculovirus cells
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: The MCP1 Rat protein solution (0.5mg/ml) contains 10% glycerol and Phosphate-Buffered Saline (pH7.4).; Sterile filtered colorless solution.
• Sequence: ADPQPDAVNA PLTCCYSFTG KMIPMSRLEN YKRITSSRCP KEAVVFVTKL KREICADPNK EWVQKYIRKL DQNQVRSETT VFYKIASTLR TSAPLNVNLT HKSEANASTL FSTTTSSTSV EVTSMTENHH HHHH

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks.; Store, frozen at -20 degree C for longer periods of time.; For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Avoid multiple freeze-thaw cycles.

Related Product Information for CCL2 recombinant protein

Introduction: Chemokine (C-C motif) ligand 2 (CCL2) is a small cytokine belongs to the CC chemokine family which is also known as monocyte chemotactic protein-1 (MCP-1). MCP 1 is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclasts. CCL2 recruits immune cells, such as monocytes, to sites of tissue injury and infection. MCP 1is produced as a protein precursor containing signal peptide of 23 amino acids and a mature peptide of 76 amino acids. As with many other CC chemokines, CCL2 is located on chromosome 17 in humans. The cell surface receptors that bind CCL2 are CCR2 and CCR5.
Description: MCP1 Rat Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 134 amino acids (24-148 aa) and having a molecular mass of 15.1kDa.
MCP1 is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.

Product Categories/Family for CCL2 recombinant protein

CHEMOKINES

NCBI and Uniprot Product Information

• NCBI GI #: 13928714
• NCBI GeneID: 24770
• NCBI Accession #: NP_113718.1
• NCBI GenBank Nucleotide #: NM_031530.1
• UniProt Accession #: P14844; Q549R5
• Molecular Weight: 16,460 Da
• NCBI Official Full Name: C-C motif chemokine 2
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 2
• NCBI Official Symbol: Ccl2
• NCBI Official Synonym Symbols: MCP-1; Scya2; Sigje
• NCBI Protein Information: C-C motif chemokine 2; immediate-early serum-responsive JE protein; immediate-early serum-responsive protein JE; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic protein 1; small inducible cytokine A2; small ind
• UniProt Protein Name: C-C motif chemokine 2
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl2
• UniProt Synonym Gene Names: Je; Mcp1; Scya2; MCP-1
• UniProt Entry Name: CCL2_RAT

NCBI Description

a monocyte chemoattractant protein [RGD, Feb 2006]

Uniprot Description

CCL13: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens. By IL1/interleukin-1 and TNF. Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Secreted; Chemokine; Motility/polarity/chemotaxis

Cellular Component: extracellular space; cell soma; rough endoplasmic reticulum; perinuclear region of cytoplasm; endocytic vesicle; dendrite; cytoplasm; extracellular region; perikaryon; synapse; nerve terminal

Molecular Function: heparin binding; G-protein-coupled receptor binding; CCR2 chemokine receptor binding; chemokine activity

Biological Process: positive regulation of cell adhesion; maternal process involved in pregnancy; positive regulation of leukocyte migration; response to glucocorticoid stimulus; response to lipopolysaccharide; response to antibiotic; monocyte chemotaxis; regulation of cell shape; response to vitamin B3; transforming growth factor beta receptor signaling pathway; negative regulation of neuron apoptosis; response to drug; neutrophil chemotaxis; organ regeneration; response to amino acid stimulus; positive regulation of tumor necrosis factor production; response to ethanol; response to bacterium; cellular response to insulin stimulus; response to heat; response to mechanical stimulus; positive regulation of endothelial cell proliferation; response to activity; response to progesterone stimulus; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of collagen biosynthetic process; chemokinesis; positive regulation of synaptic transmission; positive regulation of cellular extravasation; positive regulation of cell-cell adhesion; response to wounding; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; response to gamma radiation; inflammatory response; lymphocyte chemotaxis; aging; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; cytoskeleton organization and biogenesis; glial cell migration; macrophage chemotaxis; leukocyte migration during inflammatory response; cellular calcium ion homeostasis; negative regulation of angiogenesis; positive regulation of leukocyte mediated cytotoxicity; maternal process involved in parturition; response to hypoxia; positive regulation of T cell activation; vascular endothelial growth factor receptor signaling pathway; astrocyte cell migration