LEC (CCL16) Recombinant Protein | LEC recombinant protein

Human LEC (CCL16) Recombinant

Cat. Num. AAA553045

• Purity: >98%, as determined by SDS-PAGE and HPLC
• Synonyms: LEC (CCL16); Human LEC (CCL16) Recombinant; Human HCC-4 (CCL16) Recombinant; ccl-16 ; CCL16; hcc4; hcc-4; ncc-4; ncc4; lec; LEC recombinant protein

• Host: E Coli
• Purity/Purification: >98%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant HCC-4 was lyophilized from a 0.2 um filtered 20mM PB,100mM NaCl solution pH 7.5.
• Sequence: MKVSEAALSL LVLILIITSA SRSQPKVPEW VNTPSTCCLK YYEKVLPRRL VVGYRKALNC HLPAIIFVTK RNREVCTNPN DDWVQEYIKD PNLPLLPTRN LSTVKIITAK NGQPQLLNSQ
• Sequence Length: 120

• Domain: IL8
• Host Note: Optimized DNA sequence encoding Human LEC (CCL16) mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Human LEC, generated by the proteolytic removal of the signal peptide; and propeptide and has a calculated molecular mass of approximately 11 kDa.; ; Recombinant LEC/CCL16 is a disulfide-linked monomeric protein consisting of 98 amino acid residue subunits and migrates as an approximately 11 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for LEC recombinant protein

CCL16, also known as liver- expressed chemokine or NCC-4 or lymphocyte and monocyte chemoattractant or HCC-4, is a CC chemokine that specifically attracts lymphocytes, dendritic cells, and monocytes; increases their adhesive properties; and has myelosuppressive activity. It is constitutively expressed in liver and is increased by interleukin 10 (IL-10) in activated monocytes.CCL16 is present in human plasma suggesting that it may be active outside hepatic tissue. CCR1, CCR2, CCR5, and CCR8 are the functional receptors of this chemokine.

Product Categories/Family for LEC recombinant protein

Cytokines

References

• CCL16/lec powerfully triggers effector and antigen-presenting functions of macrophages and enhances t cell cytotoxicity .J. Leukoc. Biol., Jan 2004; 75: 135.
• CCL16 activates an angiogenic program in vascular endothelial cells .Blood, Jan 2004; 103: 40 - 49.