LEC/CCL16 Recombinant Protein | CCL16 recombinant protein

LEC/CCL16 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120314

• Gene Names: CCL16; LEC; LMC; NCC4; CKb12; HCC-4; LCC-1; Mtn-1; NCC-4; SCYL4; ILINCK; SCYA16
• Purity: >90% as determined by SDS-PAGE
• Synonyms: LEC/CCL16; LEC/CCL16 Protein; Human; Recombinant (His Tag); Human CCL16/HCC-4/NCC4 Protein (His Tag); CCL16 Protein; CKb12 Protein; HCC-4 Protein; ILINCK Protein; LCC-1 Protein; LEC Protein; LMC Protein; Mtn-1 Protein; NCC-4 Protein; NCC4 Protein; SCYA16 Protein; SCYL4 Protein; chemokine (C-C motif) ligand 16; CCL16 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gln24-Gln120

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human CCL16 (NP_004581.1)(Gln24-Gln120) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CCL16 recombinant protein

Background: CCL16, a chemokine poorly characterized at the functional level. Human CCL16 is a member of the CC family, and its gene maps to human chromosome 17q. In the mouse, only a pseudogene has been identified to date. CCL16 is a functional ligand for CCR1, CCR2, CCR5, and CCR8. Recombinant CCL16 demonstrated chemotactic activity on human monocytes and lymphocytes. Based on the ability of human chemokines to exert activity on and bind to murine receptors, the TSA mouse adenocarcinoma cell line was transfected with human CCL16 cDNA and, in comparison with other cytokines, was shown to be the faster inducer of systemic immune response due to massive, prompt infiltration of leukocytes.

References

Guiducci C, Di Carlo E, Parenza M, et al. Intralesional injection of adenovirus encoding CC chemokine ligand 16 inhibits mammary tumor growth and prevents metastatic-induced death after surgical removal of the treated primary tumor[J]. The Journal of Immunology, 2004, 172(7): 4026-4036.

NCBI and Uniprot Product Information

• NCBI GI #: 4759074
• NCBI GeneID: 6360
• NCBI Accession #: NP_004581.1
• NCBI GenBank Nucleotide #: NM_004590.3
• UniProt Accession #: O15467; Q4KKU0
• Molecular Weight: 13,600 Da
• NCBI Official Full Name: C-C motif chemokine 16
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 16
• NCBI Official Symbol: CCL16
• NCBI Official Synonym Symbols: LEC; LMC; NCC4; CKb12; HCC-4; LCC-1; Mtn-1; NCC-4; SCYL4; ILINCK; SCYA16
• NCBI Protein Information: C-C motif chemokine 16; IL-10-inducible chemokine; chemokine LEC; liver CC chemokine-1; liver-expressed chemokine; lymphocyte and monocyte chemoattractant; monotactin-1; new CC chemokine 4; small inducible cytokine subfamily A (Cys-Cys), member 16; small-
• UniProt Protein Name: C-C motif chemokine 16
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL16
• UniProt Synonym Gene Names: ILINCK; NCC4; SCYA16; HCC-4; LMC; MTN-1
• UniProt Entry Name: CCL16_HUMAN

NCBI Description

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. This cytokine also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. The expression of this gene is upregulated by IL-10. [provided by RefSeq, Jul 2008]

Uniprot Description

CCL16: Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Chemokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; chemoattractant activity

Biological Process: cell-cell signaling; positive chemotaxis; cell communication; immune response; chemotaxis; inflammatory response