Typical Testing Data/Standard Curve (for reference only)
Rabbit Caveolin-1 ELISA Kit | Cav-1 elisa kit
Rabbit Caveolin-1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CAV-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CAV-1. Standards or samples are then added to the microtiter plate wells and CAV-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CAV-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CAV-1 are added to each well to "sandwich" the CAV-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CAV-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAV-1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
Caveolin-1: May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Involved in the costimulatory signal essential for T-cell receptor (TCR)- mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3- dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Homooligomer. Interacts with GLIPR2, NOSTRIN, SNAP25 and syntaxin. Interacts with rotavirus A NSP4. Interacts (via the N- terminus) with DPP4; the interaction is direct. Interacts with CTNNB1, CDH1 and JUP. Interacts with BMX and BTK. Expressed in muscle and lung, less so in liver, brain and kidney. Belongs to the caveolin family. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: Adaptor/scaffold; Nuclear receptor co-regulator; Motility/polarity/chemotaxis
Cellular Component: Golgi apparatus; peroxisomal membrane; cell surface; mitochondrion; basolateral plasma membrane; integral to plasma membrane; integral to membrane; lipid particle; cytosol; acrosomal membrane; lipid raft; membrane; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; basal plasma membrane; cytoplasmic vesicle; intracellular; endosome; protein complex; focal adhesion; cell; endoplasmic reticulum; cell cortex; caveola; cilium; Golgi membrane; plasma membrane
Molecular Function: protein binding, bridging; identical protein binding; protease activator activity; protein kinase binding; SNARE binding; protein binding; enzyme binding; syntaxin binding; protein heterodimerization activity; protein complex scaffold; structural molecule activity; nitric-oxide synthase binding; kinase binding; receptor binding
Biological Process: negative regulation of MAP kinase activity; mammary gland involution; negative regulation of epithelial cell differentiation; positive regulation of endocytosis; negative regulation of tyrosine phosphorylation of Stat5 protein; nitric oxide homeostasis; negative regulation of BMP signaling pathway; calcium ion homeostasis; sequestering of lipid; protein localization; regulation of the force of heart contraction by chemical signal; regulation of fatty acid metabolic process; negative regulation of protein binding; regulation of the force of heart contraction; inactivation of MAPK activity; microtubule polymerization; maintenance of cellular protein localization; regulation of smooth muscle contraction; skeletal muscle development; cytosolic calcium ion homeostasis; negative regulation of nitric-oxide synthase activity; positive regulation of signal transduction; endocytosis; protein oligomerization; membrane depolarization; negative regulation of neuron differentiation; cholesterol homeostasis; response to mechanical stimulus; response to estrogen stimulus; T cell costimulation; negative regulation of endothelial cell proliferation; negative regulation of protein ubiquitination; positive regulation of endothelial cell proliferation; response to calcium ion; response to progesterone stimulus; negative regulation of JAK-STAT cascade; lactation; wound healing; negative regulation of smooth muscle cell proliferation; positive regulation of microtubule polymerization; negative regulation of peptidyl-serine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of pinocytosis; negative regulation of cell proliferation; mammary gland development; calcium ion transport; negative regulation of cytokine and chemokine mediated signaling pathway; angiogenesis; vasculogenesis; protein homooligomerization; vasoconstriction; negative regulation of MAPKKK cascade; negative regulation of nitric oxide biosynthetic process; MAPKKK cascade; cholesterol efflux; positive regulation of metalloenzyme activity; positive regulation of peptidyl-serine phosphorylation; regulation of peptidase activity; negative regulation of signal transduction; cellular calcium ion homeostasis; triacylglycerol metabolic process; regulation of blood coagulation; positive regulation of vasoconstriction; response to hypoxia
Research Articles on Cav-1
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Product Notes
The Rabbit Cav-1 cav1 (Catalog #AAA732771) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA732771 ELISA Kit recognizes Rabbit Cav-1. It is sometimes possible for the material contained within the vial of "Caveolin-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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