Typical Testing Data/Standard Curve (for reference only)
Canine Caveolin-1, Cav-1 ELISA Kit | Cav-1 elisa kit
Canine Caveolin-1, Cav-1 ELISA Kit
Gene Names
Cav1; Cav; Cav-1
Reactivity
Canine
Synonyms
Caveolin-1; Cav-1; Canine Caveolin-1; Cav-1 ELISA Kit; Cav-1 elisa kit
Reactivity
Canine
Sequence Length
147
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
20pg/ml - 6000pg/ml
Sensitivity
10.68pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Cav-1 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Canine Caveolin-1 (also known as Cav-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine Cav-1 antibody. Cav-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine Cav-1 Antibody is added and binds to Cav-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Cav-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine Cav-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine Cav-1 antibody. Cav-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine Cav-1 Antibody is added and binds to Cav-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Cav-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine Cav-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,090 Da
NCBI Official Full Name
caveolin-1 isoform 2
NCBI Official Synonym Full Names
caveolin 1, caveolae protein
NCBI Official Symbol
Cav1
NCBI Official Synonym Symbols
Cav; Cav-1
NCBI Protein Information
caveolin-1
UniProt Protein Name
Caveolin-1
UniProt Gene Name
Cav1
UniProt Synonym Gene Names
Cav
Uniprot Description
Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation (). Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway (PubMed:10816572). Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation (). Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae (PubMed:19546242).
Research Articles on Cav-1
Similar Products
Product Notes
The Canine Cav-1 cav1 (Catalog #AAA166048) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA166048 ELISA Kit recognizes Canine Cav-1. It is sometimes possible for the material contained within the vial of "Caveolin-1, Cav-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
