Rabbit anti-Human SYVN1 Polyclonal Antibody | anti-SYVN1 antibody

SYVN1 (Synovial Apoptosis Inhibitor 1, E3 Ubiquitin-protein Ligase Synoviolin, DER3, HRD1, KIAA1810) (HRP)

Cat. Num. AAA6395811

• Gene Names: SYVN1; DER3; HRD1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SYVN1; Polyclonal Antibody; SYVN1 (Synovial Apoptosis Inhibitor 1; E3 Ubiquitin-protein Ligase Synoviolin; DER3; HRD1; KIAA1810) (HRP); EC=6.3.2.-; anti-SYVN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SYVN1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SYVN1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human SYVN1, aa1-617 (NP_757385.1).
• Immunogen Sequence: MFRTAVMMAASLALTGAVVAHAYYLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKVMGKVFFGQLRAAEMEHLLERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLAEDRVDFMERSPNISWLFHCRIVSLMFLLGILDFLFVSHAYHSILTRGASVQLVFGFEYAILMTMVLTIFIKYVLHSVDLQSENPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMIKVHTFPLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEELQAMDNVCIICREEMVTGAKRLPCNHIFHTSCLRSWFQRQQTCPTCRMDVLRASLPAQSPPPPEPADQGPPPAPHPPPLLPQPPNFPQGLLPPFPPGMFPLWPPMGPFPPVPPPPSSGEAVAPPSTSAAALSRPSGAATTTAAGTSATAASATASGPGSGSAPEAGPAPGFPFPPPWMGMPLPPPFAFPPMPVPPAGFAGLTPEELRALEGHERQHLEARLQSLRNIHTLLDAAMLQINQYLTVLASLGPPRPATSVNSTEETATTVVAAASSTSIPSSEATTPTPGASPPAPEMERPPAPESVGTEEMPEDGEPDAAELRRRRLQKLESPVAH
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SYVN1 rabbit polyclonal antibody. Western Blot analysis of SYVN1 expression in HeLa.)

Western Blot (WB)

(Western Blot analysis of SYVN1 expression in transfected 293T cell line by SYVN1 polyclonal antibody. Lane 1: SYVN1 transfected lysate (67.7kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-SYVN1 antibody

Antibodies; Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 27436927
• NCBI GeneID: 84447
• NCBI Accession #: NP_757385.1
• NCBI GenBank Nucleotide #: NM_172230.2
• UniProt Accession #: Q86TM6; Q8N3K3; Q8N6E8; Q96JL5; Q96PK3
• Molecular Weight: 61,732 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase synoviolin isoform b
• NCBI Official Synonym Full Names: synoviolin 1
• NCBI Official Symbol: SYVN1
• NCBI Official Synonym Symbols: DER3; HRD1
• NCBI Protein Information: E3 ubiquitin-protein ligase synoviolin
• UniProt Protein Name: E3 ubiquitin-protein ligase synoviolin
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: SYVN1
• UniProt Synonym Gene Names: HRD1; KIAA1810

NCBI Description

This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. Sequence analysis identified two transcript variants that encode different isoforms. [provided by RefSeq, May 2011]

Uniprot Description

Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation (PubMed:12459480, PubMed:12646171, PubMed:12975321, PubMed:14593114, PubMed:16289116, PubMed:16847254, PubMed:17059562, PubMed:17141218, PubMed:17170702, PubMed:22607976, PubMed:26471130). Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (PubMed:12459480, PubMed:12646171, PubMed:12975321, PubMed:14593114, PubMed:16289116, PubMed:16847254, PubMed:17059562, PubMed:17141218, PubMed:17170702, PubMed:22607976, PubMed:26471130). Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation (PubMed:17141218). Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis (PubMed:17170702). Mediates the ubiquitination and subsequent degradation of cytoplasmic NFE2L1 ().