Mouse Slit Homolog 2 (Slit2) RTU ELISA Kit | Slit2 rtu elisa kit

Mouse Slit Homolog 2 (Slit2) ELISA Kit

Cat. Num. AAA4502538

• Gene Names: Slit2; Slil3; Drad-1; slit-2; mKIAA4141; b2b1200.1Clo; E030015M03Rik; E130320P19Rik
• Reactivity: Mouse
• Synonyms: Slit Homolog 2 (Slit2); Mouse Slit Homolog 2 (Slit2) ELISA Kit; SLIL3; Slit2 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 1521

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 78.1-5000pg/mL
• Sensitivity: 34pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Slit2 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Slit2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Slit2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Slit2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Slit2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 166064058
• NCBI GeneID: 20563
• NCBI Accession #: NP_848919.3
• NCBI GenBank Nucleotide #: NM_178804.5
• UniProt Accession #: Q9R1B9; Q9Z166; E9QKB4
• Molecular Weight: 168,783 Da
• NCBI Official Full Name: slit homolog 2 protein isoform c
• NCBI Official Synonym Full Names: slit guidance ligand 2
• NCBI Official Symbol: Slit2
• NCBI Official Synonym Symbols: Slil3; Drad-1; slit-2; mKIAA4141; b2b1200.1Clo; E030015M03Rik; E130320P19Rik
• NCBI Protein Information: slit homolog 2 protein
• UniProt Protein Name: Slit homolog 2 protein
• Protein Family: Slit homolog 2 protein
• UniProt Gene Name: Slit2
• UniProt Synonym Gene Names: Slit-2

NCBI Description

The protein encoded by this gene is a member of the Slit family of secreted glycoproteins, which function as ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. In mammals, members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Mice deficient for this gene exhibit abnormal axonal projections in the embryonic forebrain and develop supernumerary uretic buds that maintain improper connections to the nephric duct. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development, may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system, appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration ().