Human catalase ELISA Kit | CAT elisa kit

Human Catalase, Cat ELISA Kit

Cat. Num. AAA703074

• Reactivity: Human
• Synonyms: catalase; Human Catalase; Cat ELISA Kit; Human Catalase (CAT) ELISA kit; MGC138422; MGC138424; ; CAT elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CAT. No significant cross-reactivity or interference between human CAT and analogues was observed.
• Sequence Length: 527

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: < 3.9 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAT elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAT bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4557014
• NCBI GeneID: 847
• NCBI Accession #: NP_001743.1
• NCBI GenBank Nucleotide #: NM_001752.3
• UniProt Accession #: P04040; Q2M1U4; Q4VXX5; Q9BWT9; Q9UC85; A8K6C0; B2RCZ9; D3DR07
• Molecular Weight: 59,756 Da
• NCBI Official Full Name: catalase
• NCBI Official Synonym Full Names: catalase
• NCBI Official Symbol: CAT
• NCBI Protein Information: catalase
• UniProt Protein Name: Catalase
• Protein Family: Catalase
• UniProt Gene Name: CAT
• UniProt Entry Name: CATA_HUMAN

NCBI Description

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

Function: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.1 PublicationManual assertion based on experiment in:Ref.11

Catalytic activity: 2 H2O2 = O2 + 2 H2O.1 PublicationManual assertion based on experiment in:Ref.11

Cofactor: Heme group.