Caspase 14 (CASP14) Recombinant Protein | CASP14 recombinant protein

Recombinant Caspase 14 (CASP14)

Cat. Num. AAA2011161

• Gene Names: Casp14; MICE; mini-ICE
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Caspase 14 (CASP14); Recombinant Caspase 14 (CASP14); CASP14 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-MESEMSDPQP LQEERYDMSG ARLALTLCVT KAREGSEVDM EALERMFRYL KFESTMKRDP TAQQFLEELD EFQQTIDNWE EPVSCAFVVL MAHGEEGLLK GEDEKMVRLE DLFEVLNNKN CKALRGKPKV YIIQACRGEH RDPGEELRGN EELGGDEELG GDEVAVLKNN PQSIPTYTDT LHIYSTVEGY LSYRHDEKGS GFIQTLTDVF IHKKGSILEL TEEITRLMAN TEVMQEGKPR KVNPEVQSTL RKKLYLQ
• Sequence Length: 257

• Applicable Applications for CASP14 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Met1~Gln257 (Accession # O89094) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6753280
• NCBI GeneID: 12365
• NCBI Accession #: NP_033939.1
• NCBI GenBank Nucleotide #: NM_009809.5
• UniProt Accession #: O89094
• Molecular Weight: 56.5kDa
• NCBI Official Full Name: caspase-14
• NCBI Official Synonym Full Names: caspase 14
• NCBI Official Symbol: Casp14
• NCBI Official Synonym Symbols: MICE; mini-ICE
• NCBI Protein Information: caspase-14; CASP-14
• UniProt Protein Name: Caspase-14
• Protein Family: Caspase
• UniProt Gene Name: Casp14
• UniProt Synonym Gene Names: CASP-14; MICE
• UniProt Entry Name: CASPE_MOUSE

Uniprot Description

CASP14: Believed to be a non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and cornification. Probably regulates maturation of the epidermis by proteolytically processing filaggrin. Complex of unprocessed caspase-14 and processed 19 kDa (p19) and 10 kDa (p10) subunits. In undifferentiated keratinocytes under postconfluency growth conditions (in vitro). Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum). Expressed in keratinocytes of hair shaft and sebaceous glands. In psoriatic skin only expressed at very low levels. Belongs to the peptidase C14A family.

Protein type: Protease; EC 3.4.22.-

Cellular Component: cytoplasm; keratin filament; nucleus

Molecular Function: peptidase activity; hydrolase activity; cysteine-type peptidase activity

Biological Process: caspase activation; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; cell differentiation; proteolysis