Rabbit anti-Mouse ARHGEF16 Polyclonal Antibody | anti-ARHGEF16 antibody

ARHGEF16, NT (ARHGEF16, EPHEXIN4, NBR, Rho guanine nucleotide exchange factor 16, Ephexin-4) (Biotin)

Cat. Num. AAA6274964

• Gene Names: ARHGEF16; NBR; GEF16
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ARHGEF16; Polyclonal Antibody; NT (ARHGEF16; EPHEXIN4; NBR; Rho guanine nucleotide exchange factor 16; Ephexin-4) (Biotin); Ephexin-4; anti-ARHGEF16 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ARHGEF16 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ARHGEF16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-214 amino acids from the N-terminal region of human ARHGEF16.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ARHGEF16 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ARHGEF16 antibody detected the ARHGEF16 protein (arrow).)

Related Product Information for anti-ARHGEF16 antibody

Although the specific function of this protein is not known yet, it is thought to be involved in protein-protein and protein-lipid interactions.

Product Categories/Family for anti-ARHGEF16 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Hiramoto-Yamaki, N., et al. J. Cell Biol. 190(3):461-477(2010). Takefuji, M., et al. J. Hum. Genet. 55(1):42-49(2010). Barber, M.J., et al. PLoS ONE 5 (3), E9763 (2010): Rabizadeh, S., et al. Cytokine Growth Factor Rev. 14 (3-4), 225-239 (2003): Salehi, A.H., et al. J. Biol. Chem. 277(50):48043-48050(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 163792208
• NCBI GeneID: 27237
• NCBI Accession #: NP_055263.2
• NCBI GenBank Nucleotide #: NM_014448.3
• UniProt Accession #: Q5VV41; Q86TF0; Q99434
• Molecular Weight: 80,105 Da
• NCBI Official Full Name: rho guanine nucleotide exchange factor 16
• NCBI Official Synonym Full Names: Rho guanine nucleotide exchange factor (GEF) 16
• NCBI Official Symbol: ARHGEF16
• NCBI Official Synonym Symbols: NBR; GEF16
• NCBI Protein Information: rho guanine nucleotide exchange factor 16; ephexin-4; Rho guanine exchange factor (GEF) 16
• UniProt Protein Name: Rho guanine nucleotide exchange factor 16
• Protein Family: Rho guanine nucleotide exchange factor
• UniProt Gene Name: ARHGEF16
• UniProt Synonym Gene Names: EPHEXIN4; NBR
• UniProt Entry Name: ARHGG_HUMAN

NCBI Description

Although the specific function of this protein is not known yet, it is thought to be involved in protein-protein and protein-lipid interactions. [provided by RefSeq, Jul 2008]

Uniprot Description

ARHGEF16: a Rho guanine exchange factor protein that contains 1 DH, 1 PH and 1 SH3 domain.

Protein type: GEFs; Motility/polarity/chemotaxis; GEFs, Rac/Rho

Chromosomal Location of Human Ortholog: 1p36.3

Cellular Component: cytosol

Molecular Function: protein binding; Rho guanyl-nucleotide exchange factor activity; Rho GTPase binding; receptor tyrosine kinase binding; PDZ domain binding

Biological Process: regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction