Typical Testing Data/Standard Curve
Canine Chordin (CHRD) ELISA Kit | CHRD elisa kit
Canine Chordin (CHRD) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||CHRD ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CHRD antibody and an CHRD -HRP conjugate. The assay sample and buffer are incubated together with CHRD -HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHRD concentration since CHRD from samples and CHRD -HRP conjugate compete for the anti-CHRD antibody binding site. Since the number of sites is limited, as more sites are occupied by CHRD from the sample, fewer sites are left to bind CHRD -HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHRD concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a secreted protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. The encoded protein may also have roles in organogenesis and during adulthood. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their full length sequence has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
CHRD: Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes. Belongs to the chordin family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Cell adhesion
Chromosomal Location of Human Ortholog: 3q27
Cellular Component: extracellular space
Molecular Function: heparin binding; syndecan binding; protein binding; cytokine binding
Biological Process: osteoblast differentiation; BMP signaling pathway in spinal cord dorsal-ventral patterning; floor plate development; positive regulation of cell adhesion; mesoderm formation; positive regulation of mesenchymal cell proliferation; forebrain development; negative regulation of osteoblast differentiation; gastrulation with mouth forming second; negative regulation of cell migration; skeletal development; negative regulation of BMP signaling pathway
Research Articles on CHRD
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Product Notes
The Canine CHRD chrd (Catalog #AAA7238916) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7238916 ELISA Kit recognizes Canine CHRD. It is sometimes possible for the material contained within the vial of "Chordin (CHRD), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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