Sheep Gastric Inhibitory Polypeptide ELISA Kit | GIP elisa kit

Sheep Gastric Inhibitory Polypeptide ELISA Kit

Cat. Num. AAA743431

• Reactivity: Sheep
• Synonyms: Gastric Inhibitory Polypeptide; Sheep Gastric Inhibitory Polypeptide ELISA Kit; GIP elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This GIP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep GIP. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GIP elisa kit

Principle of the Assay: GIP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GIP antibody and an GIP-HRP conjugate. The assay sample and buffer are incubated together with GIP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GIP concentration since GIP from samples and GIP-HRP conjugate compete for the anti-GIP antibody binding site. Since the number of sites is limited, as more sites are occupied by GIP from the sample, fewer sites are left to bind GIP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GIP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GIP elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 4758436
• NCBI GeneID: 2695
• NCBI Accession #: NP_004114.1
• NCBI GenBank Nucleotide #: NM_004123.2
• Molecular Weight: 17,108 Da
• NCBI Official Full Name: gastric inhibitory polypeptide preproprotein
• NCBI Official Synonym Full Names: gastric inhibitory polypeptide
• NCBI Official Symbol: GIP
• NCBI Protein Information: gastric inhibitory polypeptide; incretin hormone; glucose-dependent insulinotropic polypeptide
• UniProt Protein Name: Gastric inhibitory polypeptide
• Protein Family: Gastric inhibitory polypeptide
• UniProt Gene Name: GIP
• UniProt Synonym Gene Names: GIP
• UniProt Entry Name: GIP_HUMAN

NCBI Description

This gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion. [provided by RefSeq, Jul 2008]

Uniprot Description

GIP: Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion. Belongs to the glucagon family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21.3-q22

Cellular Component: extracellular space; cell soma; endoplasmic reticulum lumen; extracellular region

Molecular Function: hormone activity

Biological Process: response to drug; response to peptide hormone stimulus; adult locomotory behavior; positive regulation of insulin secretion; female pregnancy; response to amino acid stimulus; response to lipid; sensory perception of pain; signal transduction; response to organic cyclic substance; endocrine pancreas development; memory; response to starvation; response to selenium ion; cellular protein metabolic process; response to glucose stimulus; response to axon injury; regulation of insulin secretion