Typical Testing Data/Standard Curve (for reference only)
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Canine Cartilage intermediate layer protein 1 (CILP) ELISA Kit | CILP elisa kit
Canine Cartilage intermediate layer protein 1 (CILP) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
()
Typical Testing Data/Standard Curve (for reference only)
()
Principle of the Assay: CILP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CILP1 antibody and an CILP1-HRP conjugate. The assay sample and buffer are incubated together with CILP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CILP1 concentration since CILP1 from samples and CILP1-HRP conjugate compete for the anti-CILP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CILP1 from the sample, fewer sites are left to bind CILP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CILP1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins; an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease. [provided by RefSeq, Sep 2010]
Uniprot Description
CILP: Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage. Defects in CILP are a cause of susceptibility to intervertebral disk disease (IDD). A common musculo- skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Susceptibility to intervertebral disk disease, is conferred by variant p.Ile395Thr (PubMed:15864306).
Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 15q22
Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space
Molecular Function: nucleotide diphosphatase activity; alkaline phosphatase activity
Biological Process: negative regulation of insulin-like growth factor receptor signaling pathway
Disease: Intervertebral Disc Disease
Research Articles on CILP
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Product Notes
The Canine CILP cilp (Catalog #AAA7209801) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7209801 ELISA Kit recognizes Canine CILP. It is sometimes possible for the material contained within the vial of "Cartilage intermediate layer protein 1 (CILP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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