Rabbit Cyclic adenosine monophosphate ELISA Kit | cAMP elisa kit

Rabbit Cyclic adenosine monophosphate (cAMP) ELISA Kit

Cat. Num. AAA284995

• Gene Names: CREB1; CREB
• Reactivity: Rabbit
• Synonyms: Cyclic adenosine monophosphate; Rabbit Cyclic adenosine monophosphate (cAMP) ELISA Kit; c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate; cAMP elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rabbit cAMP. No significant cross-reactivity or interference between Rabbit cAMP and analogues was observed.
• Sequence Length: 341

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for cAMP elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate cAMP in samples. An antibody specific for cAMP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anycAMP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cAMP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cAMP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 19745184
• NCBI GeneID: 1385
• NCBI Accession #: NP_604391.1
• NCBI GenBank Nucleotide #: NM_134442.3
• Molecular Weight: 25,445 Da
• NCBI Official Full Name: cyclic AMP-responsive element-binding protein 1 isoform B
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 1
• NCBI Official Symbol: CREB1
• NCBI Official Synonym Symbols: CREB
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 1
• UniProt Protein Name: Cyclic AMP-responsive element-binding protein 1
• Protein Family: Cathelicidin antimicrobial peptide
• UniProt Gene Name: CREB1
• UniProt Synonym Gene Names: CREB-1; cAMP-responsive element-binding protein 1
• UniProt Entry Name: CREB1_HUMAN

NCBI Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CREB: a transcription factor of the leucine zipper family of DNA binding proteins. Binds as a homodimer to the cAMP-responsive element (CRE). Phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: axon; mitochondrial matrix; nucleoplasm; nucleus

Molecular Function: enzyme binding; histone acetyltransferase binding; Hsp70 protein binding; identical protein binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding; transcription cofactor activity; transcription factor activity

Biological Process: aging; axon guidance; circadian rhythm; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; lactation; memory; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; nerve growth factor receptor signaling pathway; Notch signaling pathway; phosphoinositide-mediated signaling; phospholipase C activation; pituitary gland development; positive regulation of apoptosis; positive regulation of fat cell differentiation; positive regulation of hormone secretion; positive regulation of lipid biosynthetic process; positive regulation of multicellular organism growth; positive regulation of osteoclast differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transcriptional preinitiation complex assembly; positive regulation of transforming growth factor-beta3 production; protein amino acid phosphorylation; protein stabilization; regulation of cell size; regulation of circadian rhythm; regulation of fibroblast proliferation; response to activity; response to glucagon stimulus; response to hypoxia; response to nicotine; response to organic substance; secretory granule organization and biogenesis; signal transduction; stress-activated MAPK cascade; synaptic transmission; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription from RNA polymerase II promoter; transforming growth factor beta receptor signaling pathway; viral reproduction; visual learning

Disease: Histiocytoma, Angiomatoid Fibrous