Mouse anti-Human RHOT1 Polyclonal Antibody | anti-RHOT1 antibody

RHOT1 (Ras Homolog Gene Family Member T1, ARHT1, Mitochondrial Rho GTPase 1, MIRO1, MIRO-1, hMiro-1, Rac-GTP-binding Protein-like Protein)

Cat. Num. AAA649092

• Gene Names: RHOT1; ARHT1; MIRO1; MIRO-1
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RHOT1; Polyclonal Antibody; RHOT1 (Ras Homolog Gene Family Member T1; ARHT1; Mitochondrial Rho GTPase 1; MIRO1; MIRO-1; hMiro-1; Rac-GTP-binding Protein-like Protein); Anti -RHOT1 (Ras Homolog Gene Family Member T1; anti-RHOT1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RHOT1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHISDGVADSGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFKVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAVTVTRDKKIDLQKKQTQRNVFRCNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHARLRCMCTCNRCTFCICQNFLNSDLLQSVKNKIFTAVLNRHVTQADLKSSTFWLRASFGATVFAVLGFAMYKALLKQR

• Applicable Applications for anti-RHOT1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human RHOT1, aa1-659 (NP_001028738.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of RHOT1 expression in transfected 293T cell line by RHOT1 polyclonal antibody. Lane 1: RHOT1 transfected lysate (72.49kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-RHOT1 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 46249998
• NCBI GeneID: 55288
• UniProt Accession #: Q8IXI2; Q6NUR3; Q6P9F8; Q6PJG1; Q6YMW8; Q86UB0; Q8IW28; Q8IXJ7; A4FVB6; A6NFV0; B4DG48; J9JIH9
• Molecular Weight: 70,784 Da
• NCBI Official Full Name: RHOT1 protein, partial
• NCBI Official Synonym Full Names: ras homolog family member T1
• NCBI Official Symbol: RHOT1
• NCBI Official Synonym Symbols: ARHT1; MIRO1; MIRO-1
• NCBI Protein Information: mitochondrial Rho GTPase 1; hMiro-1; mitochondrial Rho 1; mitochondrial Rho (MIRO) GTPase 1; ras homolog gene family, member T1; rac-GTP binding protein-like protein; rac-GTP-binding protein-like protein
• UniProt Protein Name: Mitochondrial Rho GTPase 1
• UniProt Gene Name: RHOT1
• UniProt Synonym Gene Names: ARHT1; MIRO-1; hMiro-1
• UniProt Entry Name: MIRO1_HUMAN

Uniprot Description

Function: Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution. Ref.2 Ref.9

Subunit structure: Interacts with the kinesin-binding proteins TRAK1/OIP106 and TRAK2/GRIF1, forming a link between mitochondria and the trafficking apparatus of the microtubules. Ref.9

Subcellular location: Mitochondrion outer membrane; Single-pass type IV membrane protein. Note: Colocalizes with MGARP and RHOT2 at the mitochondria. Ref.2 Ref.10

Tissue specificity: Ubiquitously expressed. Expressed at high level in heart and skeletal muscle. Ref.2

Sequence similarities: Belongs to the mitochondrial Rho GTPase family.Contains 2 EF-hand domains.Contains 2 Miro domains.

Sequence caution: The sequence AAH41114.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH68463.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA91969.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB14185.1 differs from that shown. Reason: Erroneous initiation.