Mouse Cyclic Adenosine Monophosphate (cAMP) ELISA Kit | cAMP elisa kit

Mouse Cyclic Adenosine Monophosphate (cAMP) ELISA Kit

Cat. Num. AAA265450

• Gene Names: CREB1; CREB
• Reactivity: Mouse
• Synonyms: Cyclic Adenosine Monophosphate (cAMP); Mouse Cyclic Adenosine Monophosphate (cAMP) ELISA Kit; cAMP elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.
• Sequence Length: 341

• Samples: Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant cAMP concentration.
• Assay Type: Sandwich
• Detection Range: 200 pmol/ml-3.12 pmol/ml
• Sensitivity: 0.6 pmol/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Related Product Information for cAMP elisa kit

Principle of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse cAMP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 19745184
• NCBI GeneID: 1385
• NCBI Accession #: NP_604391.1
• NCBI GenBank Nucleotide #: NM_134442.3
• Molecular Weight: 25,445 Da
• NCBI Official Full Name: cyclic AMP-responsive element-binding protein 1 isoform B
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 1
• NCBI Official Symbol: CREB1
• NCBI Official Synonym Symbols: CREB
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 1; CREB-1; transactivator protein; active transcription factor CREB; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1
• UniProt Protein Name: Cyclic AMP-responsive element-binding protein 1
• Protein Family: Cathelicidin antimicrobial peptide
• UniProt Gene Name: CREB1
• UniProt Synonym Gene Names: CREB-1; cAMP-responsive element-binding protein 1
• UniProt Entry Name: CREB1_HUMAN

NCBI Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CREB: a transcription factor of the leucine zipper family of DNA binding proteins. Binds as a homodimer to the cAMP-responsive element (CRE). Phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: nucleoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; enzyme binding; transcription cofactor activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; circadian rhythm; lactation; axon guidance; response to glucagon stimulus; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; positive regulation of multicellular organism growth; signal transduction; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; response to organic substance; toll-like receptor 5 signaling pathway; synaptic transmission; toll-like receptor 4 signaling pathway; epidermal growth factor receptor signaling pathway; response to drug; mitochondrion organization and biogenesis; Notch signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; protein stabilization; secretory granule organization and biogenesis; organelle organization and biogenesis; MyD88-independent toll-like receptor signaling pathway; positive regulation of hormone secretion; positive regulation of lipid biosynthetic process; memory; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; phospholipase C activation; pituitary gland development; toll-like receptor signaling pathway; positive regulation of fat cell differentiation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; regulation of cell size

Disease: Histiocytoma, Angiomatoid Fibrous