Immumohistochemistry (IHC)
(Immunochemical staining CASQ1 in human skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Rabbit Calsequestrin 1 Polyclonal Antibody | anti-CASQ1 antibody
Anti-Calsequestrin 1 Antibody, Rabbit Polyclonal
Reactivity
Human, Cynomolgus
Applications
ELISA, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
Calsequestrin 1; Polyclonal Antibody; Anti-Calsequestrin 1 Antibody; Rabbit Polyclonal; Calsequestrin-1/CASQ1 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-CASQ Antibody; Anti-PDIB1 Antibody; Anti-VMCQA Antibody; calsequestrin 1 (fast-twitch; skeletal muscle); anti-CASQ1 antibody
Host
Rabbit
Reactivity
Human, Cynomolgus
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Calsequestrin 1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid. 0.2um filtered solution in PBS
Applicable Applications for anti-CASQ1 antibody
ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
IHC-P: 1:500-1:2000
Immunogen
Recombinant Human Calsequestrin-1/CASQ1 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human Calsequestrin-1/CASQ1 (rh Calsequestrin-1/CASQ1; P31415; Gln35-Asp396). Calsequestrin-1/CASQ1 specific IgG was purified by Human Calsequestrin-1/CASQ1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Immumohistochemistry (IHC)
(Immunochemical staining CASQ1 in human skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immumohistochemistry (IHC)
(Immunochemical staining CASQ1 in human skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining CASQ1 in cynomolgus heart with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining CASQ1 in cynomolgus heart with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-CASQ1 antibody
Background: Calsequestrin-1 is an isoform of calsequestrin. Calsequestrin is a calcium-binding protein of the sarcoplasmic reticulum. It helps hold calcium in the cisterna of the sarcoplasmic reticulum after a muscle contraction, even though the concentration of calcium in the sarcoplasmic reticulum is much higher than in the cytosol. Two forms of calsequestrin have been identified: Calsequestrin-2 and Calsequestrin-1. Calsequestrin-1 is found in fast skeletal muscle. The release of calsequestrin-bound calcium (through a calcium release channel) triggers muscle contraction. The active protein is not highly structured, more than 5% of it adopting a random coil conformation. When calcium binds there is a structural change whereby the alpha-helical content of the protein increases from 3 to 11%. Both forms of calsequestrin are phosphorylated by casein kinase 2, but the cardiac form is phosphorylated more rapidly and to a higher degree. Calsequestrin-1 is also secreted in the gut where it deprives bacteria of calcium ions.
References
Slupsky JR, et al. (1987) Characterization of cardiac calsequestrin. Biochemistry. 26(20): 6539-44. Cala SE, et al. (1991) Phosphorylation of cardiac and skeletal muscle calsequestrin isoforms by casein kinase II. Demonstration of a cluster of unique rapidly phosphorylated sites in cardiac calsequestrin. J Biol Chem. 266(1):391-8. Wang S, et al. (1998) Crystal structure of calsequestrin from rabbit skeletal muscle sarcoplasmic reticulum. Nat Struct Biol. 5(6):476-83.
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Product Notes
The CASQ1 (Catalog #AAA8119065) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Calsequestrin 1 Antibody, Rabbit Polyclonal reacts with Human, Cynomolgus and may cross-react with other species as described in the data sheet. AAA Biotech's Calsequestrin 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). ELISA: 1:5000-1:10,000 IHC-P: 1:500-1:2000. Researchers should empirically determine the suitability of the CASQ1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calsequestrin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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