Typical Testing Data/Standard Curve
Rat Calbindin (CALB1) ELISA Kit | CALB1 elisa kit
Rat Calbindin (CALB1) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||CALB1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CALB1 antibody and an CALB1-HRP conjugate. The assay sample and buffer are incubated together with CALB1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CALB1 concentration since CALB1 from samples and CALB1-HRP conjugate compete for the anti-CALB1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CALB1 from the sample, fewer sites are left to bind CALB1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CALB1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The neurons in brains of patients with Huntington disease are calbindin-depleted. [provided by RefSeq, Jul 2008]
Uniprot Description
CALB1: Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Interacts with RANBP9. Belongs to the calbindin family.
Protein type: Calcium-binding
Chromosomal Location of Human Ortholog: 8q21.3
Cellular Component: cell soma; axon; dendrite; synapse; cytosol; nucleus
Molecular Function: vitamin D binding; protein binding; zinc ion binding; calcium ion binding
Biological Process: cytosolic calcium ion homeostasis; long-term memory; short-term memory; regulation of synaptic plasticity; locomotory behavior
Research Articles on CALB1
Similar Products
Product Notes
The Rat CALB1 calb1 (Catalog #AAA7244639) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7244639 ELISA Kit recognizes Rat CALB1. It is sometimes possible for the material contained within the vial of "Calbindin (CALB1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
