Western Blot (WB)
(Western blot analysis of ATG101 in human liver tissue lysate with ATG101 antibody at (A) 1 and (B) 2 μg/mL.)
Rabbit ATG101 Polyclonal Antibody | anti-C12orf44 antibody
ATG101 Antibody
Gene Names
ATG101; C12orf44
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
ATG101 Antibody is affinity chromatography purified via peptide column.
Synonyms
ATG101; Polyclonal Antibody; ATG101 Antibody; C12orf44; PP894; Autophagy-related protein 101; chromosome 12 open reading frame 44; anti-C12orf44 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
ATG101 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
218
Applicable Applications for anti-C12orf44 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
ATG101 antibody can be used for detection of ATG101 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
ATG101 antibody was raised against a 16 amino acid synthetic peptide near the center of human ATG101.
Buffer
ATG101 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
ATG101 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of ATG101 in human liver tissue lysate with ATG101 antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB)
(Western blot analysis of ATG101 in human liver tissue lysate with ATG101 antibody at (A) 1 and (B) 2 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of ATG101 in mouse liver with ATG101 antibody at 5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of ATG101 in mouse liver with ATG101 antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ATG101 in mouse liver tissue with ATG101 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ATG101 in mouse liver tissue with ATG101 antibody at 20 μg/mL.)
Related Product Information for anti-C12orf44 antibody
ATG101 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG101 is a recently discovered protein that stabilizes ATG13, another autophagy protein that forms a complex with the mammalian homologs of ATG1, ULK1 and ULK2, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. ATG101 also interacts with ULK1 and is essential for autophagy.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,003 Da
NCBI Official Full Name
autophagy-related protein 101
NCBI Official Synonym Full Names
autophagy related 101
NCBI Official Symbol
ATG101
NCBI Official Synonym Symbols
C12orf44
NCBI Protein Information
autophagy-related protein 101; Atg13-interacting protein
UniProt Protein Name
Autophagy-related protein 101
UniProt Gene Name
ATG101
UniProt Synonym Gene Names
C12orf44
UniProt Entry Name
ATGA1_HUMAN
Uniprot Description
ATG101: an protein required for autophagosome formation. Interacts with ATG13. Associates with a complex composed of ATG13, ULK1 and RB1CC1. Under starvation conditions it localizes to phagophores, which are preautophagosomal structures. Stabilizes the expression of Atg13 in the cell. Belongs to the ATG101 family.
Protein type: Autophagy
Chromosomal Location of Human Ortholog: 12q13.13
Molecular Function: identical protein binding; protein binding; protein complex binding
Biological Process: autophagic vacuole formation
Research Articles on C12orf44
Similar Products
Product Notes
The C12orf44 atg101 (Catalog #AAA150402) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATG101 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATG101 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). ATG101 antibody can be used for detection of ATG101 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the C12orf44 atg101 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG101, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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