Typical Testing Data/Standard Curve (for reference only)
Chicken Calcium Binding Protein D9K ELISA Kit | CABPD9k elisa kit
Chicken Calcium Binding Protein D9K ELISA Kit
Gene Names
S100g; CABP1; Calb3; Cabp9k; AU042539; CaBP-D9K
Reactivity
Chicken
Synonyms
Calcium Binding Protein D9K; Chicken Calcium Binding Protein D9K ELISA Kit; CABPD9k elisa kit
Reactivity
Chicken
Specificity
This assay has high sensitivity and excellent specificity for detection of D9K. No significant cross-reactivity or interference between D9K and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between D9K and all the analogues, therefore, cross reaction may still exist in some cases.
Sequence Length
79
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Reactivity Note
No significant cross-reactivity or interference between this antigen and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection, therefore, cross reaction may still exist in some cases.
Preparation and Storage
Store at 2-8 degree C.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CABPD9k elisa kit
Intended Uses: This D9K ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken D9K. This ELISA kit for research use only!
Principle of the Assay: D9K ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-D9K antibody and an D9K-HRP conjugate. The assay sample and buffer are incubated together with D9K-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the D9K concentration since D9K from samples and D9K-HRP conjugate compete for the anti-D9K antibody binding site. Since the number of sites is limited, as more sites are occupied by D9K from the sample, fewer sites are left to bind D9K-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The D9K concentration in each sample is interpolated from this standard curve.
Principle of the Assay: D9K ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-D9K antibody and an D9K-HRP conjugate. The assay sample and buffer are incubated together with D9K-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the D9K concentration since D9K from samples and D9K-HRP conjugate compete for the anti-D9K antibody binding site. Since the number of sites is limited, as more sites are occupied by D9K from the sample, fewer sites are left to bind D9K-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The D9K concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
8,970 Da
NCBI Official Full Name
calcium-binding protein D9k
NCBI Official Synonym Full Names
S100 calcium binding protein G
NCBI Official Symbol
S100g
NCBI Official Synonym Symbols
CABP1; Calb3; Cabp9k; AU042539; CaBP-D9K
NCBI Protein Information
protein S100-G
UniProt Protein Name
Protein S100-G
UniProt Gene Name
S100g
UniProt Synonym Gene Names
Calb3; S100d; CABP
Research Articles on CABPD9k
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Product Notes
The Chicken CABPD9k s100g (Catalog #AAA7264620) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7264620 ELISA Kit recognizes Chicken CABPD9k. It is sometimes possible for the material contained within the vial of "Calcium Binding Protein D9K, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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