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Typical Testing Data/Standard Curve (for reference only) ()

Mouse Endostatin (ES) ELISA Kit | ES elisa kit

Mouse Endostatin (ES) ELISA Kit

Reactivity
Mouse
Synonyms
Endostatin (ES); Mouse Endostatin (ES) ELISA Kit; ES elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
No cross-reaction with other factors.
Sequence Length
184
Samples
Mouse serum, plasma or cell culture supernatant
Assay Type
Quantitative Sandwich
Detection Range
10 ng/mL-0.156 ng/mL
Sensitivity
Up to 0.05 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

()

Typical Testing Data/Standard Curve (for reference only) ()
Related Product Information for ES elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse ES monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
134,204 Da
NCBI Official Full Name
endostatin, partial
NCBI Official Synonym Full Names
collagen, type XVIII, alpha 1
NCBI Official Symbol
Col18a1
NCBI Protein Information
collagen alpha-1(XVIII) chain; collagen alpha-1(XVIII) chain; endostatin; procollagen, type XVIII, alpha 1
UniProt Protein Name
Collagen alpha-1(XVIII) chain
UniProt Gene Name
Col18a1
UniProt Entry Name
COIA1_MOUSE

Uniprot Description

COL18A1: COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Defects in COL18A1 are a cause of Knobloch syndrome type 1 (KNO1). An autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele. Belongs to the multiplexin collagen family. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; collagen; extracellular region; basement membrane

Molecular Function: identical protein binding; metal ion binding; structural molecule activity

Biological Process: extracellular matrix organization and biogenesis; positive regulation of cell proliferation; endothelial cell morphogenesis; angiogenesis; cell adhesion; positive regulation of cell migration

Research Articles on ES

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Product Notes

The Mouse ES col18a1 (Catalog #AAA266186) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA266186 ELISA Kit recognizes Mouse ES. It is sometimes possible for the material contained within the vial of "Endostatin (ES), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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