CA8 recombinant protein

CA8, 1-290aa, Human, His tag, E Coli

Cat. Num. AAA204726

• Gene Names: CA8; CALS; CARP; CA-RP; CAMRQ3; CA-VIII
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: CA8; 1-290aa; Human; His tag; E Coli; Carbonic anhydrase-related protein; CA-VIII; CALS; CAMRQ3; CARP; CA8 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 1mM DTT
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMADLSF IEDTVAFPEK EEDEEEEEEG VEWGYEEGVE WGLVFPDANG EYQSPINLNS REARYDPSLL DVRLSPNYVV CRDCEVTNDG HTIQVILKSK SVLSGGPLPQ GHEFELYEVR FHWGRENQRG SEHTVNFKAF PMELHLIHWN STLFGSIDEA VGKPHGIAII ALFVQIGKEH VGLKAVTEIL QDIQYKGKSK TIPCFNPNTL LPDPLLRDYW VYEGSLTIPP CSEGVTWILF RYPLTISQLQ IEEFRRLRTH VKGAELVEGC DGILGDNFRP TQPLSDRVIR AAFQ
• Sequence Length: 290

• Applicable Applications for CA8 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CA8 recombinant protein

CA8 was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, this protein lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). It continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. Defects in CA8 are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 3 (CMARQ3). Recombinant human CA8 protein fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for CA8 recombinant protein

Cancer

References

Turkmen S., et al. (2009) PLoS Genet. 5:E1000487-E1000487; Skaggs L.A., et al. (1993) Gene. 126:291-292

NCBI and Uniprot Product Information

• NCBI GI #: 22027500
• NCBI GeneID: 767
• NCBI Accession #: NP_004047
• NCBI GenBank Nucleotide #: NM_004056.5
• UniProt Accession #: P35219; Q32MY2; A8K0A5; B3KQZ7
• Molecular Weight: 35.5 kDa (314aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: carbonic anhydrase-related protein isoform a
• NCBI Official Synonym Full Names: carbonic anhydrase 8
• NCBI Official Symbol: CA8
• NCBI Official Synonym Symbols: CALS; CARP; CA-RP; CAMRQ3; CA-VIII
• NCBI Protein Information: carbonic anhydrase-related protein
• UniProt Protein Name: Carbonic anhydrase-related protein
• Protein Family: Carbonic anhydrase-related protein
• UniProt Gene Name: CA8
• UniProt Synonym Gene Names: CALS; CARP; CA-VIII

NCBI Description

The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. Mutations in this gene are associated with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CMARQ3). Polymorphisms in this gene are associated with osteoporosis, and overexpression of this gene in osteosarcoma cells suggests an oncogenic role. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

Does not have a carbonic anhydrase catalytic activity.