Mouse RBP4 (Retinol Binding Protein 4, Plasma) CLIA Kit | RBP4 clia kit

Mouse RBP4 (Retinol Binding Protein 4, Plasma) CLIA Kit

Cat. Num. AAA2532888

• Gene Names: RBP4; RDCCAS; MCOPCB10
• Reactivity: Mouse
• Synonyms: RBP4 (Retinol Binding Protein 4; Plasma); Mouse RBP4 (Retinol Binding Protein 4; Plasma) CLIA Kit; RBP-4; PRBP; RBP4 clia kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinant RBP4. No significant cross-reactivity or interference between RBP4 and analogues was observed.
• Sequence Length: 201

• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RBP4 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of RBP4 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to RBP4. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for RBP4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain RBP4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of RBP4. You can calculate the concentration of RBP4 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18088326
• NCBI GeneID: 5950
• Molecular Weight: 23,010 Da
• NCBI Official Full Name: Retinol binding protein 4, plasma
• NCBI Official Synonym Full Names: retinol binding protein 4, plasma
• NCBI Official Symbol: RBP4
• NCBI Official Synonym Symbols: RDCCAS; MCOPCB10
• NCBI Protein Information: retinol-binding protein 4
• UniProt Protein Name: Retinol-binding protein 4
• Protein Family: Retinol-binding protein
• UniProt Gene Name: RBP4
• UniProt Synonym Gene Names: PRBP; RBP
• UniProt Entry Name: RET4_HUMAN

NCBI Description

This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq, Jul 2008]

Uniprot Description

RBP4: Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency). This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q23.33

Cellular Component: extracellular space; protein complex; extracellular region; cytosol

Molecular Function: protein binding; protein heterodimerization activity; retinol binding; retinal binding

Biological Process: cardiac muscle development; phototransduction, visible light; response to retinoic acid; urinary bladder development; embryonic skeletal development; maintenance of gastrointestinal epithelium; heart development; vagina development; positive regulation of insulin secretion; positive regulation of immunoglobulin secretion; glucose homeostasis; uterus development; gluconeogenesis; embryonic organ morphogenesis; response to ethanol; visual perception; eye development; negative regulation of cardiac muscle cell proliferation; retinol metabolic process; embryonic retina morphogenesis in camera-type eye; retinoid metabolic process; female genitalia morphogenesis; lung development

Disease: Retinal Dystrophy, Iris Coloboma, And Comedogenic Acne Syndrome; Microphthalmia, Isolated, With Coloboma 10