Mouse Complement component C8 gamma chain ELISA Kit | C8g elisa kit

Mouse Complement component C8 gamma chain ELISA Kit

Cat. Num. AAA2880129

• Reactivity: Mouse
• Synonyms: Complement component C8 gamma chain; Mouse Complement component C8 gamma chain ELISA Kit; C8g; C8g elisa kit

• Reactivity: Mouse
• Sequence Length: 202

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Related Product Information for C8g elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 58037159
• NCBI GeneID: 69379
• NCBI Accession #: NP_081338.1
• NCBI GenBank Nucleotide #: NM_027062.2
• UniProt Accession #: Q8VCG4
• Molecular Weight: 22,508 Da
• NCBI Official Full Name: complement component C8 gamma chain isoform 1
• NCBI Official Synonym Full Names: complement component 8, gamma polypeptide
• NCBI Official Symbol: C8g
• NCBI Protein Information: complement component C8 gamma chain
• UniProt Protein Name: Complement component C8 gamma chain
• Protein Family: Complement component
• UniProt Gene Name: C8g
• UniProt Entry Name: CO8G_MOUSE

NCBI Description

This gene encodes a secreted protein that is a core component of the complement 8 (C8) complex. C8 is part of the membrane attack complex which participates in the innate immune response against bacterial pathogens. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]

Uniprot Description

C8G: C8 is a constituent of the membrane attack complex. C8 binds to the C5B-7 complex, forming the C5B-8 complex. C5-B8 binds C9 and acts as a catalyst in the polymerization of C9. The gamma subunit seems to be able to bind retinol. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular region; extracellular space; membrane attack complex

Molecular Function: complement binding; protein complex binding; retinol binding

Biological Process: complement activation, alternative pathway; complement activation, classical pathway; cytolysis; immune system process; innate immune response