Human Deoxyribonuclease gamma ELISA Kit | DNASE1L3 elisa kit

Human Deoxyribonuclease gamma ELISA Kit

Cat. Num. AAA2881557

• Gene Names: DNASE1L3; LSD; DHP2; SLEB16; DNAS1L3
• Reactivity: Human
• Synonyms: Deoxyribonuclease gamma; Human Deoxyribonuclease gamma ELISA Kit; DNase gamma; DNase I homolog protein DHP2; Deoxyribonuclease I-like 3; DNase I-like 3; Liver and spleen DNase; LS-DNase; LSD; DNASE1L3; DHP2; DNASE1L3 elisa kit

• Reactivity: Human
• Sequence Length: 275

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DNASE1L3 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 375151549
• NCBI GeneID: 1776
• NCBI Accession #: NP_001243489.1
• NCBI GenBank Nucleotide #: NM_001256560.1
• UniProt Accession #: Q13609; O75803; B2R8B1; B7Z707
• Molecular Weight: 31,860 Da
• NCBI Official Full Name: deoxyribonuclease gamma isoform 2
• NCBI Official Synonym Full Names: deoxyribonuclease I-like 3
• NCBI Official Symbol: DNASE1L3
• NCBI Official Synonym Symbols: LSD; DHP2; SLEB16; DNAS1L3
• NCBI Protein Information: deoxyribonuclease gamma
• UniProt Protein Name: Deoxyribonuclease gamma
• Protein Family: Deoxyribonuclease
• UniProt Gene Name: DNASE1L3
• UniProt Synonym Gene Names: DHP2; DNAS1L3; DNase gamma; DNase I-like 3; LS-DNase; LSD
• UniProt Entry Name: DNSL3_HUMAN

NCBI Description

This gene encodes a member of the deoxyribonuclease I family. The encoded protein hydrolyzes DNA, is not inhibited by actin, and mediates the breakdown of DNA during apoptosis. Mutations in this gene are a cause of systemic lupus erythematosus-16. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

DNASE1L3: Has DNA hydrolytic activity. Does not bind to actin. Cleaves chromatin DNA to nucleosomal units. Defects in DNASE1L3 are the cause of systemic lupus erythematosus type 16 (SLEB16). A rare autosomal recessive form of systemic lupus erythematosus with childhood onset, characterized by high frequency of anti-neutrophil cytoplasmic antibodies and lupus nephritis. Systemic lupus erythematosus is a chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow. Belongs to the DNase I family.

Protein type: Secreted, signal peptide; Calcium-binding; Secreted; Apoptosis; Deoxyribonuclease; EC 3.1.21.-

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: nucleus

Molecular Function: calcium ion binding; deoxyribonuclease activity; DNA binding; endodeoxyribonuclease activity, producing 5'-phosphomonoesters

Biological Process: DNA fragmentation during apoptosis; DNA metabolic process

Disease: Systemic Lupus Erythematosus 16