Rabbit IL-5 Polyclonal Antibody | anti-IL5 antibody

RABBIT ANTI HUMAN INTERLEUKIN-5

Cat. Num. AAA221338

• Gene Names: IL5; EDF; TRF; IL-5
• Applications: ELISA, Flow Cytometry, Functional Assay, Functional Assay, Western Blot
• Synonyms: IL-5; Polyclonal Antibody; RABBIT ANTI HUMAN INTERLEUKIN-5; anti-IL5 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0mg/ml after reconstitution (varies by lot)
• Sequence Length: 134

• Applicable Applications for anti-IL5 antibody: ELISA (EIA), Flow cytometry (FC/FACS), Functional Assays (FN), Western Blot (WB)
• Application Notes: ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml;; Functional Assays: Minimum Dilution: 0.05; Maximum Dilution: 0.09ug/ml;; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
• Immunogen: Recombinant human IL-5; Buffer Solution
• Antiserum Preparation: Antisera to human IL-5 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Staining of formalin fixed, paraffin embedded human colo-rectal carcinoma with Rabbit anti Human interleukin-5 (MBS221338))

Related Product Information for anti-IL5 antibody

Rabbit anti Human Interleukin-5 antibody recognizes human IL-5 (Interleukin-5), a disulphide-linked homodimer consisting of two 115 amino acid chains, which is homologous to mouse eosinophil differentiation factor (EDF) and is produced by mast cells, eosinophils and T cells. IL-5 is a lineage-specific cytokine involved in the development and stimulation of eosinophils, and has been linked with diseases involving abnormally high levels of eosinophils, including parasitic infections and allergic airways eosinophilia, the major pathological symptom of allergic asthma.

NCBI and Uniprot Product Information

• NCBI GI #: 4504671
• NCBI GeneID: 3567
• NCBI Accession #: NP_000870.1
• NCBI GenBank Nucleotide #: NM_000879.2
• UniProt Accession #: P05113; Q13840
• Molecular Weight: 15,238 Da
• NCBI Official Full Name: interleukin-5
• NCBI Official Synonym Full Names: interleukin 5
• NCBI Official Symbol: IL5
• NCBI Official Synonym Symbols: EDF; TRF; IL-5
• NCBI Protein Information: interleukin-5; B-cell differentiation factor I; T-cell replacing factor; colony-stimulating factor, eosinophil; eosinophil differentiation factor
• UniProt Protein Name: Interleukin-5
• Protein Family: Interleukin
• UniProt Gene Name: IL5
• UniProt Synonym Gene Names: IL-5; TRF
• UniProt Entry Name: IL5_HUMAN

NCBI Description

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]

Uniprot Description

IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.

Protein type: Secreted; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; interleukin-5 receptor binding; growth factor activity; cytokine activity

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of B cell proliferation; positive regulation of JAK-STAT cascade; positive regulation of transcription factor activity; immune response; positive regulation of immunoglobulin secretion; inflammatory response; positive regulation of eosinophil differentiation