gamma-Glutamyl Transferase Active Protein | GGT1 active protein

Gamma-Glutamyl Transferase (gGT), Bovine Kidney

• Gene Names: GGT1; GGT; GTG; CD224; GGT 1; D22S672; D22S732
• Synonyms: gamma-Glutamyl Transferase; Gamma-Glutamyl Transferase (gGT); Bovine Kidney; gamma-Glutamyl Transpeptidase; gGT; GGT1 active protein

• Host: Bovine Kidney
• Form/Format: Powder, lyophilized from tris-acetate, pH 7.0
• Sequence Length: 225

• CAS Number: 9046-27-9
• E.C. Number: 2.3.2.2
• Appearance: Tan Powder
• Activity: 15 U/mg (Dimension® Clinical Chemistry System)
• Unit Definition: One unit will catalyze the transfer of one micromole of the glutamyl moiety from gamma-glutamyl-3-carboxy-4-nitranilide to glycylglycine per minute at 37°C.
• Protein: 0.43 mg protein/mg solid (Coomassie); 0.59 mg protein/mg solid(A₂₈₀)
• Specific Activity: 36 U/mg protein (Coomassie); 26 U/mg protein (A₂₈₀)
• Contaminants: CPK: < 1.0%; LDH: < 1.0%; SGOT/AST: < 1.0% ; ALP: < 0.2%; SGPT/ALT: < 0.01%
• Ammonia: < 0.1 umole/mg
• Reconstitution: > 1 mg/ml in tris buffered saline, 1% BSA, pH 8.0. Aliquot and freeze at -20°C after reconstitution.
• Handling: Handle as a potentially hazardous substance.
• Preparation and Storage: Ships on blue ice.; Store at -20°C. Protect from moisture.

Related Product Information for GGT1 active protein

MyBioSource is the largest manufacturer of Bovine Kidney gamma-Glutamyl Transpeptidase, Gamma-glutamyl transferase (gGT/GGTP). Single batch sizes of 100,000 kU.

Custom preparations, technical support, bulk quantities and aliquoting available.

Detection of liver disease, obstructive jaundice, cholangitis, infectious hepititus, and cholecystitis. gamma-Glutamyltransferase (gGT/GGTP) test is used to detect diseases of the liver, bile ducts, and kidney; and to differentiate liver or bile duct (hepatobiliary) disorders from bone disease.

Our Bovine Kidney gamma-Glutamyl Transpeptidase (gGT/GGTP) is used in multi-analyte clinical chemistry controls and calibrators for major IVD manufacturers around the world. We are able to custom purify Bovine Kidney gGT to meet your specific application.

Product Categories/Family for GGT1 active protein

Proteins; Antigens; Enzymes; Standards/controls; Native Proteins; Gamma-glutamyl Transferase (ggt)

NCBI and Uniprot Product Information

• NCBI GI #: 73915096
• NCBI GeneID: 2678
• NCBI Accession #: NP_001027537.1
• NCBI GenBank Nucleotide #: NM_001032365.2
• NCBI Official Synonym Full Names: gamma-glutamyltransferase 1
• NCBI Official Symbol: GGT1
• NCBI Official Synonym Symbols: GGT; GTG; CD224; GGT 1; D22S672; D22S732
• NCBI Protein Information: gamma-glutamyltranspeptidase 1
• UniProt Protein Name: Gamma-glutamyltranspeptidase 1
• Protein Family: Gamma-glutamyltranspeptidase
• UniProt Gene Name: GGT1
• UniProt Synonym Gene Names: GGT; GGT 1
• UniProt Entry Name: GGT1_HUMAN

NCBI Description

The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014]

Uniprot Description

GGT1: Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive. Defects in GGT1 are a cause of glutathionuria (GLUTH); also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease. Belongs to the gamma-glutamyltransferase family. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - glutathione; EC 2.3.2.2; Other Amino Acids Metabolism - selenoamino acid; Transferase; Membrane protein, integral; EC 3.4.19.14; Other Amino Acids Metabolism - cyanoamino acid; Lipid Metabolism - arachidonic acid; EC 3.4.19.13

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: anchored to external side of plasma membrane; extracellular space; plasma membrane; integral to membrane

Molecular Function: protein binding; gamma-glutamyltransferase activity

Biological Process: amino acid metabolic process; glutamate metabolic process; regulation of immune system process; cysteine biosynthetic process; leukotriene metabolic process; proteolysis; leukotriene biosynthetic process; glutathione metabolic process; xenobiotic metabolic process; regulation of inflammatory response; glutathione catabolic process; glutathione biosynthetic process; arachidonic acid metabolic process; spermatogenesis; zymogen activation

Disease: Glutathionuria