Western Blot (WB)
(Figure 1. Western blot analysis of NRXN3 using anti-NRXN3 antibody (MBS1753713).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-NRXN3 antigen affinity purified polyclonal antibody (Catalog # MBS1753713) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for NRXN3 at approximately 185KD. The expected band size for NRXN3 is at 185KD. )
Rabbit NRXN3 Polyclonal Antibody | anti-NRXN3 antibody
Anti-NRXN3 Antibody
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of NRXN3 using anti-NRXN3 antibody (MBS1753713).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-NRXN3 antigen affinity purified polyclonal antibody (Catalog # MBS1753713) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for NRXN3 at approximately 185KD. The expected band size for NRXN3 is at 185KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of NRXN3 using anti-NRXN3 antibody (MBS1753713).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-NRXN3 antigen affinity purified polyclonal antibody (Catalog # MBS1753713) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for NRXN3 at approximately 185KD. The expected band size for NRXN3 is at 185KD. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of U20S cells using anti-NRXN3 antibody (MBS1753713).Overlay histogram showing U20S cells stained with MBS1753713 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NRXN3 Antibody (MBS1753713, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of U20S cells using anti-NRXN3 antibody (MBS1753713).Overlay histogram showing U20S cells stained with MBS1753713 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NRXN3 Antibody (MBS1753713, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Keum, S., Kim, A., Shin, J. J., Kim, J. -H., Park, J., Shin, H. -S. A missense variant at the Nrxn3 locus enhances empathy fear in the mouse. Neuron 98: 588-601, 2018.
3. Missler, M., Sudhof, T. C. Neurexins: three genes and 1001 products. Trends Genet. 14: 20-26, 1998.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a family of proteins that function in the nervous system as receptors and cell adhesion molecules. Extensive alternative splicing and the use of alternative promoters results in multiple transcript variants and protein isoforms for this gene, but the full-length nature of many of these variants has not been determined. Transcripts that initiate from an upstream promoter encode alpha isoforms, which contain epidermal growth factor-like (EGF-like) sequences and laminin G domains. Transcripts initiating from the downstream promoter encode beta isoforms, which lack EGF-like sequences. Genetic variation at this locus has been associated with a range of behavioral phenotypes, including alcohol dependence and autism spectrum disorder. [provided by RefSeq, Dec 2012]
Uniprot Description
NRXN3B: Neuronal cell surface protein that may be involved in cell recognition and cell adhesion. May play a role in angiogenesis. {ECO:0000250}. Belongs to the neurexin family. {ECO:0000305}. The cytoplasmic C-terminal region binds to CASK. Binds to neuroligins NLGN1, NLGN2 and NLGN3. Weakly interacts with CBLN1 and CBLN2. Very weak binding, if any, with CBLN4. {ECO:0000250}. 7 isoforms of the human protein are produced by alternative promoter
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 14q31
Cellular Component: integral to plasma membrane; integral to membrane
Molecular Function: metal ion binding; calcium channel regulator activity; receptor activity; cell adhesion molecule binding
Biological Process: synaptic transmission; axon guidance; positive regulation of synaptogenesis; synaptogenesis; neurotransmitter secretion; adult behavior; neuron adhesion; learning; angiogenesis; social behavior
Research Articles on NRXN3
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Product Notes
The NRXN3 nrxn3 (Catalog #AAA1753713) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NRXN3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NRXN3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Mouse, Rat| FC/FACS/FCM: 1-3ug/1x106 cells|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the NRXN3 nrxn3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NRXN3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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