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Typical Testing Data/Standard Curve (for reference only)

Human gamma-glutamyltransferase 1 ELISA Kit | GGT1 elisa kit

Human gamma glutamyl transpeptidase, GGT ELISA Kit

Gene Names
GGT1; GGT; GTG; CD224; GGT 1; D22S672; D22S732
Reactivity
Human
Synonyms
gamma-glutamyltransferase 1; Human gamma glutamyl transpeptidase; GGT ELISA Kit; CD224; D22S672; D22S732; GGT; GTG; MGC96892; MGC96904; MGC96963; OTTHUMP00000028921; OTTHUMP00000159078; gamma-glutamyl transpeptidase; glutamyl transpeptidase; GGT1 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human GGT1. No significant cross-reactivity or interference between Human GGT1 and analogues was observed.
Sequence Length
569
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Biosynthesis/Metabolism
Protein Biological Process 2
Amino-acid biosynthesis and metabolism
Protein Biological Process 3
Glutathione biosynthesis
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GGT1 elisa kit
Introduction: Gamma-glutamyl transferase (GGT or GGTP, or Gamma-GT) is involved in the transfer of amino acids across the cellular membrane. It is also involved in glutathione metabolism by transferring the glutamyl moiety to a variety of acceptor molecules including water, certain L-amino acids and peptides. Leaving the cysteine product to preserve intracellular homeostasis of oxidative stress. GGT is found on the cell surface on all cells, with particularly high concentrations in the liver, bile ducts, and kidney. The enzyme is also present in other tissues, such as the epididymis. Elevated GGT is a marker of recent alcohol misuse. It may indicate excess alcohol consumption up to 3 or 4 weeks prior to the test. It is also used as a marker for other types of liver damage or conditions attributable to other causes unrelated to alcohol abuse.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GGT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for GGT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain GGT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GGT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61,410 Da
NCBI Official Full Name
gamma-glutamyltranspeptidase 1
NCBI Official Synonym Full Names
gamma-glutamyltransferase 1
NCBI Official Symbol
GGT1
NCBI Official Synonym Symbols
GGT; GTG; CD224; GGT 1; D22S672; D22S732
NCBI Protein Information
gamma-glutamyltranspeptidase 1; glutathione hydrolase 1; leukotriene-C4 hydrolase
UniProt Protein Name
Gamma-glutamyltranspeptidase 1
UniProt Gene Name
GGT1
UniProt Synonym Gene Names
GGT; GGT 1
UniProt Entry Name
GGT1_HUMAN

NCBI Description

The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014]

Uniprot Description

Function: Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates, and other gamma-glutamyl compounds. The metabolism of glutathione releases free glutamate and the dipeptide, cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Isoform 3 seems to be inactive.7 PublicationsManual assertion based on experiment in:Ref.7

Catalytic activity: A (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid.8 PublicationsManual assertion based on experiment in:Ref.15

Enzyme regulation: Activated by autocatalytic cleavage.1 PublicationManual assertion based on experiment in:Ref.24

Research Articles on GGT1

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Product Notes

The Human GGT1 ggt1 (Catalog #AAA704964) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA704964 ELISA Kit recognizes Human GGT1. It is sometimes possible for the material contained within the vial of "gamma-glutamyltransferase 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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