Pyruvate Dehydrogenase Complex Substrate
Pyruvate Dehydrogenase Complex (PDC)
Purity
Highly Purified90% (SDS-PAGE) Subunit apparant Mr: ~74,000 (E2), 53,000 (E3), 41,000 (E1-a) and 36,000 E1-B).
Synonyms
Pyruvate Dehydrogenase Complex; Pyruvate Dehydrogenase Complex (PDC); Pyruvate Dehydrogenase Complex substrate
Host
Bovine heart
Purity/Purification
Highly Purified
90% (SDS-PAGE) Subunit apparant Mr: ~74,000 (E2), 53,000 (E3), 41,000 (E1-a) and 36,000 E1-B).
90% (SDS-PAGE) Subunit apparant Mr: ~74,000 (E2), 53,000 (E3), 41,000 (E1-a) and 36,000 E1-B).
Form/Format
Supplied as a liquid in 50mM Tris HCl, pH 7.0, 14mM b-mercaptoethanol, 1mM benzamidine, 0.1mM PMSF, 1mM EDTA and 10% glycerol
Specific Activity
~12-18umol of NADH formed per min per mg of protein with pyruvate as substrate at 30 degree C.
Preparation and Storage
For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.
Related Product Information for Pyruvate Dehydrogenase Complex substrate
Mitochondrial enzyme regulated by reversible phosphorylation. Catalyzes the oxidative decarboxylation of pyruvate. Purified preparations undergo phosphorylation because endogenous kinase is tightly associated with the complex.
Product Categories/Family for Pyruvate Dehydrogenase Complex substrate
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Product Notes
The Pyruvate Dehydrogenase Complex (Catalog #AAA639278) is a Substrate produced from Bovine heart and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Pyruvate Dehydrogenase Complex, Substrate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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