Typical Testing Data/Standard Curve (for reference only)
(Fig.1. fT4 Standard Curve.)
Free Thyroxine (fT4) ELISA Kit | fT4 elisa kit
Free Thyroxine (fT4) ELISA Kit
Reactivity
Human, Mouse, Rat, Goat, Sheep, Dog, Rabbit, Bovine, Monkey, Chicken, Fish, Swine
Synonyms
Free Thyroxine (fT4); Free Thyroxine (fT4) ELISA Kit; fT4 elisa kit
Reactivity
Human, Mouse, Rat, Goat, Sheep, Dog, Rabbit, Bovine, Monkey, Chicken, Fish, Swine
Specificity
fT4 ELISA Kit has high sensitivity and excellent specificity for detection of fT4. No significant cross-reactivity or interference between fT4 and analogues was observed.
Assay Type
Competitive ELISA (Quantitative)
Samples
Serum, Plasma, Other Biological Fluids
Detection Range
4 pmol/L-64 pmol/L
Detection Method
Colorimetric
Limit of Detection
4.0 pmol/L
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. fT4 Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. fT4 Standard Curve.)
Related Product Information for fT4 elisa kit
Description: fT4 ELISA Kit employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to fT4. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated fT4. A competitive inhibition reaction is launched between fT4 (Standards or samples) and Biotin-conjugated fT4 with the pre-coated antibody specific for fT4. The more amount of fT4 in samples, the less antibody bound by Biotin-conjugated fT4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of fT4 in the sample. The color development is stopped and the intensity of the color is measured.
Background: Over 99% of Triiodothyronine (T4) circulates in blood is bound to carrier proteins; thyroxine-binding globulin (TBG). However, only the free (unbound) portion of T4 is responsible for the biological action. Further, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In normal thyroid function as the concentrations of the carrier proteins alters, the total T4 level changes so that the free T4 concentration remains constant. Thus, measurements of free T4 concentrations correlate more reliably with clinical status than total T4 levels. The increase in total T4 levels associated with pregnancy, oral contraceptives and estrogen therapy result in higher total T4 levels while the free T4 concentration remains basically unchanged.
Background: Over 99% of Triiodothyronine (T4) circulates in blood is bound to carrier proteins; thyroxine-binding globulin (TBG). However, only the free (unbound) portion of T4 is responsible for the biological action. Further, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In normal thyroid function as the concentrations of the carrier proteins alters, the total T4 level changes so that the free T4 concentration remains constant. Thus, measurements of free T4 concentrations correlate more reliably with clinical status than total T4 levels. The increase in total T4 levels associated with pregnancy, oral contraceptives and estrogen therapy result in higher total T4 levels while the free T4 concentration remains basically unchanged.
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Product Notes
The Human, Mouse, Rat, Goat, Sheep, Dog, Rabbit, Bovine, Monkey, Chicken, Fish, Swine fT4 (Catalog #AAA9711533) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711533 ELISA Kit recognizes Human, Mouse, Rat, Goat, Sheep, Dog, Rabbit, Bovine, Monkey, Chicken, Fish, Swine fT4. It is sometimes possible for the material contained within the vial of "Free Thyroxine (fT4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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