Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Thyroxine antibody (TAb) Standard Curve.)
Rat Thyroxine (TAb) ELISA Kit | TAb elisa kit
Rat Thyroxine Antibody (TAb) ELISA Kit
Reactivity
Rat
Synonyms
Thyroxine (TAb); Rat Thyroxine Antibody (TAb) ELISA Kit; TAb elisa kit
Reactivity
Rat
Specificity
Rat Thyroxine antibody (TAb) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TAb. No significant cross-reactivity or interference between Rat TAb and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Thyroxine antibody (TAb) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Thyroxine antibody (TAb) Standard Curve.)
Related Product Information for TAb elisa kit
Description: This Rat Thyroxine antibody (TAb) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TAb. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated TAb and incubated. The competitive inhibition reaction is launched between with HRP labeled TAb and unlabeled TAb with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of TAb in the sample. The color development is stopped and the intensity of the color is measured.
Background: T4, the principal thyroid hormone largely bound to transport proteins, especially TBG. Given normal levels of thyroid hormone-binding proteins, hyperthyroidism is characterized by increased levels of circulating T4, hypothyroidism by decreased levels. Exceptions to this parallelism between thyroid status and total T4 concentration are found. Levels of TBG are known to be altered under various physiological, pharmacological and genetic conditions. Elevated T4 levels may be obtained when TBG levels are high, as in pregnancy, acute intermittent porphyria, hyperproteinemia, hereditary TBG elevation and in patients undergoing estrogen therapy or taking oral contraceptives. Total T4 levels may be depressed when TBG levels are low, as in nephrotic, hepatic, gastrointestinal and neoplastic disorders; in acromegaly, hypoproteinemia and hereditary TBG deficiency; and in patients undergoing androgen, testosterone or anabolic steroid therapy.
Background: T4, the principal thyroid hormone largely bound to transport proteins, especially TBG. Given normal levels of thyroid hormone-binding proteins, hyperthyroidism is characterized by increased levels of circulating T4, hypothyroidism by decreased levels. Exceptions to this parallelism between thyroid status and total T4 concentration are found. Levels of TBG are known to be altered under various physiological, pharmacological and genetic conditions. Elevated T4 levels may be obtained when TBG levels are high, as in pregnancy, acute intermittent porphyria, hyperproteinemia, hereditary TBG elevation and in patients undergoing estrogen therapy or taking oral contraceptives. Total T4 levels may be depressed when TBG levels are low, as in nephrotic, hepatic, gastrointestinal and neoplastic disorders; in acromegaly, hypoproteinemia and hereditary TBG deficiency; and in patients undergoing androgen, testosterone or anabolic steroid therapy.
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Product Notes
The Rat TAb (Catalog #AAA9712654) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712654 ELISA Kit recognizes Rat TAb. It is sometimes possible for the material contained within the vial of "Thyroxine (TAb), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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