Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Thyroxine-binding globulin (SERPINA7) Standard Curve.)
Mouse Thyroxine-Binding Globulin (SERPINA7) ELISA Kit | TBG elisa kit
Mouse Thyroxine-Binding Globulin (SERPINA7) ELISA Kit
Gene Names
Serpina7; Tbg; C730040N12Rik
Reactivity
Mouse
Synonyms
Thyroxine-Binding Globulin (SERPINA7); Mouse Thyroxine-Binding Globulin (SERPINA7) ELISA Kit; SERPINA7; RP1-82J11.2; OTTHUMP00000023779; alpha-1 antiproteinase; antitrypsin; serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 7; serine (or cysteine) prote; TBG elisa kit
Reactivity
Mouse
Specificity
Mouse Thyroxine-binding globulin (SERPINA7) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SERPINA7. No significant cross-reactivity or interference between Mouse SERPINA7 and analogues was observed.
Sequence Length
418
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Thyroxine-binding globulin (SERPINA7) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Thyroxine-binding globulin (SERPINA7) Standard Curve.)
Related Product Information for TBG elisa kit
Description: This Mouse Thyroxine-binding globulin (SERPINA7) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINA7 in samples. An antibody specific for SERPINA7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPINA7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Thyroxine-binding globulin (TBG) binds Thyroid hormone in circulation. It is one of three proteins (along with transthyretin and albumin) responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3'-triiodothyronine (T3) in the bloodstream. Of these three proteins, TBG has the highest affinity for T4 and T3, but is present in the lowest concentration. Despite its low concentration, TBG carries the majority of T4 in serum. Due to the very low serum concentration of T4 & T3, TBG is rarely more than 25% saturated with its ligand. Unlike transthyretin and albumin, TBG has a single binding site for T4/T3. TBG is synthesized primarily in the liver as a 54 kDa protein. Genomically, TBG is a serpin, although it has no inhibitory function like many other members of this class of proteins.
Background: Thyroxine-binding globulin (TBG) binds Thyroid hormone in circulation. It is one of three proteins (along with transthyretin and albumin) responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3'-triiodothyronine (T3) in the bloodstream. Of these three proteins, TBG has the highest affinity for T4 and T3, but is present in the lowest concentration. Despite its low concentration, TBG carries the majority of T4 in serum. Due to the very low serum concentration of T4 & T3, TBG is rarely more than 25% saturated with its ligand. Unlike transthyretin and albumin, TBG has a single binding site for T4/T3. TBG is synthesized primarily in the liver as a 54 kDa protein. Genomically, TBG is a serpin, although it has no inhibitory function like many other members of this class of proteins.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
thyroxine-binding globulin isoform X2
NCBI Official Synonym Full Names
serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
NCBI Official Symbol
Serpina7
NCBI Official Synonym Symbols
Tbg; C730040N12Rik
NCBI Protein Information
thyroxine-binding globulin
UniProt Protein Name
Thyroxine-binding globulin
UniProt Gene Name
Serpina7
UniProt Synonym Gene Names
Tbg
UniProt Entry Name
THBG_MOUSE
Uniprot Description
SERPINA7: Major thyroid hormone transport protein in serum. Defects in SERPINA7 are a cause of thyroxine-binding globulin deficiency (TBG deficiency). Mutations in the SERPINA7 gene can result as a whole spectrum of deficiencies, characterized by either reduced or increased TBG levels in the serum. Patients show, respectively, reduced or elevated protein- bound iodine but are euthyroid. Belongs to the serpin family.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular region; extracellular space
Molecular Function: hormone binding; serine-type endopeptidase inhibitor activity
Biological Process: post-embryonic development; response to drug; response to vitamin A
Research Articles on TBG
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Product Notes
The Mouse TBG serpina7 (Catalog #AAA9717061) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717061 ELISA Kit recognizes Mouse TBG. It is sometimes possible for the material contained within the vial of "Thyroxine-Binding Globulin (SERPINA7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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