ALK-3 / BMPR1A recombinant protein

Recombinant Mouse ALK-3 / BMPR1A Protein (His tag)

Cat. Num. AAA2547233

• Gene Names: Bmpr1a; ALK3; Bmpr; BMPR-IA; AU045487; 1110037I22Rik
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: ALK-3 / BMPR1A; Recombinant Mouse ALK-3 / BMPR1A Protein (His tag); Bmpr1a; 1110037I22Rik; ALK3; AU045487; BMPR-IA; Bmpr; ALK-3 / BMPR1A recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 532

• Application Notes: The recombinant mouse ALK3 consists of 140 amino acids after removal of the signal peptide and has a predicted molecular mass of 15.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm ALK3 is approximately 30 kDa due to glycosylation.
• Predicted N Terminal: Gln 24
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for ALK-3 / BMPR1A recombinant protein

Background: Activin receptor-Like Kinase 3 (ALK-3), also known as Bone Morphogenetic Protein Receptor, type IA (BMPR1A), is a type I receptor for bone morphogenetic proteins (BMPs) which belong to the transforming growth factor beta (TGF-beta) superfamily. The BMP receptors form a subfamily of transmembrane serine/threonine kinases including the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. ALK-3/BMPR1A is expressed in the epithelium during branching morphogenesis. Deletion of BMPR1A in the epithelium with an Sftpc-cre transgene leads to dramatic defects in lung development. ALK-3 and ALK-6 share a high degree of homology, yet possess distinct signaling roles. The transforming growth factor (TGF)-beta type III receptor (TbetaRIII) enhanced both ALK-3 and ALK-6 signaling. TbetaRIII associated with ALK-3 primarily through their extracellular domains, whereas its interaction with ALK-6 required both the extracellular and cytoplasmic domains. ALK-3 plays an essential role in the formation of embryonic ventral abdominal wall, and abrogation of BMP signaling activity due to gene mutations in its signaling components could be one of the underlying causes of omphalocele at birth. The type IA BMP receptor, ALK-3 was specifically required at mid-gestation for normal development of the trabeculae, compact myocardium, interventricular septum, and endocardial cushion. Cardiac muscle lacking ALK-3 was specifically deficient in expressing TGFbeta2, an established paracrine mediator of cushion morphogenesis. Hence, ALK-3 is essential, beyond just the egg cylinder stage, for myocyte-dependent functions and signals in cardiac organogenesis.

Description: A DNA sequence encoding the extracellular domain (Met 1-Arg 152) of mouse ALK3 (NP_033888.2) precursor was expressed, fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 46519168
• NCBI GeneID: 12166
• NCBI Accession #: NP_033888.2
• NCBI GenBank Nucleotide #: NM_009758.4
• UniProt Accession #: P36895
• Molecular Weight: 60,063 Da
• NCBI Official Full Name: bone morphogenetic protein receptor type-1A
• NCBI Official Synonym Full Names: bone morphogenetic protein receptor, type 1A
• NCBI Official Symbol: Bmpr1a
• NCBI Official Synonym Symbols: ALK3; Bmpr; BMPR-IA; AU045487; 1110037I22Rik
• NCBI Protein Information: bone morphogenetic protein receptor type-1A
• UniProt Protein Name: Bone morphogenetic protein receptor type-1A
• UniProt Gene Name: Bmpr1a
• UniProt Synonym Gene Names: Acvrlk3; Bmpr; BMP type-1A receptor; BMPR-1A; ALK-3; SKR5

Uniprot Description

BMPR1A: a serine/threonine-protein kinase receptor for Bone morphogenetic protein-2 and -4 (BMP-2 and BMP-4). Defects in BMPR1A are a cause of juvenile polyposis syndrome (JPS) and Cowden disease (CD), a cancer syndrome characterized by multiple hamartomas and by a high risk for breast, thyroid and endometriel cancers.

Protein type: EC 2.7.11.30; Kinase, protein; Membrane protein, integral; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; STKR family; TKL group; Type1 subfamily

Chromosomal Location of Human Ortholog: 14 B|14 20.81 cM

Cellular Component: caveola; cell soma; dendrite; external side of plasma membrane; plasma membrane

Molecular Function: ATP binding; glycoprotein binding; protein binding; protein homodimerization activity; protein serine/threonine kinase activity; SMAD binding

Biological Process: anterior/posterior pattern formation; BMP signaling pathway; cartilage development; cell differentiation; chondrocyte differentiation; developmental growth; dorsal/ventral axis specification; dorsal/ventral pattern formation; ectoderm development; embryonic digit morphogenesis; embryonic morphogenesis; embryonic organ development; endoderm development; heart development; heart morphogenesis; hindlimb morphogenesis; immune response; in utero embryonic development; lateral mesoderm development; lung development; mesendoderm development; mesoderm formation; Mullerian duct regression; negative regulation of neurogenesis; negative regulation of smooth muscle cell migration; nervous system development; neural crest cell development; neural plate mediolateral pattern formation; odontogenesis of dentine-containing teeth; palate development; paraxial mesoderm development; paraxial mesoderm structural organization; pattern specification process; pituitary gland development; positive regulation of bone mineralization; positive regulation of cardiac muscle cell proliferation; positive regulation of epithelial cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of cardiac muscle cell proliferation; regulation of endothelial cell differentiation; regulation of endothelial cell proliferation; regulation of lateral mesodermal cell fate specification; somitogenesis; stem cell maintenance